Table 2 Summary and distribution of assembled sequences. Length distribution and characteristics of contigs assembled from the two GS-20 runs, one GS-FLX run, the three 454 runs combined and from the control Sanger sequenced ESTs
From: High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome
| Â | GS-20 | GS-FLX | 454 all | Sanger (control) |
|---|---|---|---|---|
Run(s) in assembly | 1 + 2 | 3 | 1 + 2 + 3 | - |
≤ 100 bp | 18987 (29%) | 52 (<1%) | 10820 (15%) | 0 |
101 – 250 bp | 42320 (66%) | 17348 (62%) | 35958 (50%) | 0 |
251 – 500 bp | 2476 (4%) | 5355 (19%) | 18768 (26%) | 7564 (35%) |
501 – 750 bp | 535 (<1%) | 2463 (9%) | 2869 (4%) | 9226 (43%) |
751 – 1000 bp | 167 (<1%) | 1314 (5%) | 1396 (2%) | 2830 (13%) |
> 1000 bp | 99 (<1%) | 1547 (6%) | 1573 (2%) | 1812 (8%) |
Total contigs | 64584 (100%) | 28079 (100%) | 71384 (100%) | 21432 (100%) |
Average contig length (bp) | 130.6 | 353.22 | 247.16 | 623.35 |
Reads in contigs | 80.72% | 71.36% | 88.41% | 84,88% |
Average reads/contig | 7.89 | 9.98 | 12.69 | 3.42 |