Figure 8

MegaBlast analysis showing sequence identity to WGS reads using the sequence of the satellite marker BARC-Satt688 (CC454041) that anchored the BES from H77P02 (CG817343 and CG817333) to contig 9354. MegaBlast against 7.3 million reads with repeat masking gave 4 nearly identical. If the few differences were HSVs then there might be 3 homeologous sequences. Each homeolog was represented by a single read suggesting this region is under-represented in the sequence database. The ATT repeat that formed the microsatellite polymorphism was at 360–418 bp (not shown) and was 2 bp shorter in Williams 82 than Forrest. A potential SNP between Forrest and Williams 82 is circled (SIUC-SNP_Satt688).