Figure 3
From: Genetic interaction network of the Saccharomyces cerevisiae type 1 phosphatase Glc7
glc7-E101Q mutant is impaired in glucose metabolism. (A) Growth of wild type (GLC7), mutant (glc7-E101Q) and rescue (glc7-E101Q/GLC7-res) strains was examined by spotting assays (5-fold serial dilutions of 1.0 × 106 cells/ml; 5 μl/spot) grown in YPAD or YPAG for 48 hr at 30°C and 37°C. (B) Glycogen content was assessed in wild type (GLC7), mutant (glc7-E101Q) and rescue (GLC7-res) strains by iodine staining. Brown color correlates with glycogen content.