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Table 1 Composition of the culture medium.

From: Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses

Product Concentration (g/L) Product Concentration (g/L)
Glucose 20 Isoleucine 0.025
Sodium acetate 1 Leucine 0.05875
Ammonium citrate 0.6 Valine 0.04125
KH2PO4 3 (9) Cysteine 0.17
K2HPO4 2,5 (7,5) Phenylalanine 0.28
MgCl2, 6H2O 0.2 Tyrosine 0.29
FeSO4, 7H2O 0.011 Adenine 0.05
CaCl2, 2H2O 0.05 Guanine 0.05
ZnSO4, 7H2O 0.005 Uracil 0.05
COCl2, 6H2O 0.0025 Xanthin 0.05
Alanine 0.24 P-aminobenzoic acid 0.01
Arginine 0.12 Biotin 0.01
Asparagine 0.34 Cyano-cobalamine (B12) 0.001
Glutamine 0.51 Folic acid 0.01
Glycine 0.17 Inosine 0.025
Histidine 0.11 Nicotinic acid 0.001
Lysine 0.35 Orotic acid 0.005
Methionine 0.12 Ca panthotenate 0.001
Proline 0.68 Pyridoxamine 0.005
Serine 0.34 Pyridoxin (B6) 0.002
Threonine 0.23 Riboflavin (B2) 0.001
Tryptophan 0.05 Thiamine 0.001
   D, L-6,8-thioctic acid 0.0025
   Thymidine 0.025
  1. The lines separating the different products indicate those that are dissolved together. Values into brackets in front of KH2PO4 and K2HPO4 indicate the required concentrations to obtain a buffered media for pre-cultures