Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis

Nelson, Curtis M; Herron, Michael J; Felsheim, Roderick F; Schloeder, Brian R; Grindle, Suzanne M; Chavez, Adela Oliva; Kurtti, Timothy J; Munderloh, Ulrike G

doi:10.1186/1471-2164-9-364

Table 2 Summary of Ap-HL-60 vs. Ap-ISE6 differential gene transcription

From: Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis

	Gene Product	Locus	Predicted Cellular Location	Fold Change Ap-HL-60/Ap-ISE6
1	DNA-binding protein	APH_1100	Cytoplasmic	4.9
2	HGE-14 protein	APH_0387	Extracellular	4.8
3	hypothetical protein	APH_1412	Outer Membrane	4.2
4	hypothetical protein	APH_0915	Outer Membrane, Extracellular	4.1
5	hypothetical protein	APH_0906	Outer Membrane	4.1
6	major outer membrane protein OMP-1A	APH_1359	Outer Membrane	4.0
7	hypothetical protein	APH_1378	Outer Membrane	3.8
8	hypothetical protein	APH_0842	Cytoplasmic	3.7
9	hypothetical protein	APH_0838	Outer Membrane	3.6
10	hypothetical protein	APH_0388	Cytoplasmic	3.6
11	hypothetical protein	APH_1145	Inner Membrane	3.5
12	OmpA family protein	APH_0338	Outer Membrane	3.4
13	DNA-binding response regulator	APH_1099	Cytoplasmic	3.4
14	hypothetical protein	APH_1144	Inner Membrane	3.4
15	hypothetical protein	APH_0837	Cytoplasmic	3.4
16	HGE-14 protein	APH_0382	Extracellular	3.3
17	hypothetical protein	APH_0005	Inner Membrane	3.3
18	hypothetical protein	APH_0756	Inner Membrane, Cytoplasmic	3.2
19	10 kDa chaperonin	APH_0241	Periplasmic	3.2
20	hypothetical protein	APH_0032	Outer Membrane, Extracellular	3.1
21	hypothetical protein	APH_0874	Outer Membrane	3.1
22	hypothetical protein	APH_0233	Inner Membrane	3.1
23	HGE-14 protein	APH_0385	Cytoplasmic	3.1
24	signal peptidase II	APH_1160	Inner Membrane	3.0
25	hypothetical protein	APH_1156	Cytoplasmic	3.0
26	hypothetical protein	APH_0793	Inner Membrane	2.9
27	HGE-14 protein	APH_0455	Extracellular	2.9
28	Omp-1N	APH_1220	Outer Membrane	2.8
29	hypothetical protein	APH_0949	Inner Membrane, Cytoplasmic	2.7
30	hypothetical protein	APH_0033	Cytoplasmic	2.7
31	hypothetical protein	APH_1307	Inner Membrane	2.7
32	hypothetical protein	APH_1157	Inner Membrane	2.7
33	hypothetical protein	APH_1151	Inner Membrane	2.6
34	antioxidant AhpC/Tsa family	APH_0795	Cytoplasmic	2.6
35	RNA polymerase sigma-32 factor	APH_0759	Cytoplasmic	2.6
36	60 kDa chaperonin	APH_0240	Cytoplasmic	2.6
37	hypothetical protein	APH_1235	Cytoplasmic	2.5
38	hypothetical protein	APH_0922	Inner Membrane	2.5
39	hypothetical protein	APH_1262	Cytoplasmic	2.5
40	hypothetical protein	APH_0757	Cytoplasmic	2.5
41	chaperone protein DnaK	APH_0346	Cytoplasmic	2.4
42	hypothetical protein	APH_1236	Cytoplasmic	2.4
43	hypothetical protein	APH_0363	Cytoplasmic	2.4
44	translation initiation factor IF-3	APH_1263	Cytoplasmic	2.4
45	glyceraldehyde-3-phosphate dehydrogenase type I	APH_1349	Cytoplasmic	2.4
46	hypothetical protein	APH_0873	Cytoplasmic	2.4
47	hypothetical protein	APH_0919	Inner Membrane	2.4
48	hypothetical protein	APH_1072	Cytoplasmic	2.4
49	hypothetical protein	APH_1320	Cytoplasmic	2.3
50	HGE-14 protein	APH_0453	Cytoplasmic	2.3
51	outer membrane protein MSP2 family	APH_1325	Outer Membrane	2.2
52	hypothetical protein	APH_0643	Cytoplasmic	2.2
53	hypothetical protein	APH_0839	Outer Membrane	2.2
54	putative acyl carrier protein	APH_0929	Cytoplasmic	2.2
55	Es1 family protein	APH_0006	Cytoplasmic	2.2
56	hypothetical protein	APH_0179	Cytoplasmic	2.2
57	iron-sulfur cluster assembly accessory protein	APH_0676	Cytoplasmic	2.2
58	putative ATP synthase F0 B' subunit	APH_1190	Cytoplasmic	2.1
59	hypothetical protein	APH_0719	Cytoplasmic	2.1
60	hypothetical protein	APH_0991	Cytoplasmic	2.1
61	succinate dehydrogenase cytochrome b556 subunit	APH_0999	Inner Membrane	2.1
62	pyruvate phosphate dikinase	APH_0185	Cytoplasmic	2.1
63	iron-binding protein	APH_0051	Cytoplasmic	2.1
64	nucleoside diphosphate kinase	APH_1217	Cytoplasmic	2.1
65	malonyl CoA-acyl carrier protein transacylase	APH_0092	Cytoplasmic	2.0
66	hypothetical protein	APH_0786	Cytoplasmic	2.0
67	co-chaperone GrpE	APH_0036	Cytoplasmic	2.0
68	hypothetical protein	APH_0771	Cytoplasmic	2.0
69	hypothetical protein	APH_0585	Cytoplasmic	2.0
70	hypothetical protein	APH_0655	Cytoplasmic	2.0
71	ribonucleoside-diphosphate reductase alpha subunit	APH_0331	Cytoplasmic	2.0
72	P44-45 outer membrane protein	APH_0171	Outer Membrane	2.0
73	adenylosuccinate lyase	APH_0867	Cytoplasmic	2.0
74	P44-36 outer membrane protein	APH_1168	Outer Membrane	2.0
75	aspartate aminotransferase	APH_0660	Cytoplasmic	2.0
76	cytochrome C membrane-bound	APH_0180	Periplasmic	2.0
	35/76 named genes ≥ 2-fold up in Ap -HL-60 = 54% hypothetical		33/76 genes membrane associated = 43%
77	hypothetical protein	APH_0197	Periplasmic	0.5
78	hypothetical protein	APH_0369	Cytoplasmic	0.5
79	hypothetical protein	APH_0497	Cytoplasmic	0.5
80	hypothetical protein	APH_0425	Cytoplasmic	0.5
81	hypothetical protein	APH_0587	Cytoplasmic	0.5
82	hypothetical protein	APH_0963	Cytoplasmic	0.5
83	hypothetical protein	APH_1130	Inner Membrane	0.5
84	hypothetical protein	APH_0467	Cytoplasmic	0.5
85	thiamine biosynthesis protein ThiC truncation	APH_0586	Cytoplasmic	0.5
86	hypothetical protein	APH_0806	Periplasmic	0.5
87	hypothetical protein	APH_0599	Cytoplasmic	0.5
88	hypothetical protein	APH_0827	Cytoplasmic	0.4
89	outer membrane efflux protein	APH_1110	Outer Membrane	0.4
90	hypothetical protein	APH_1131	Inner Membrane	0.4
91	hypothetical protein	APH_0829	Cytoplasmic	0.4
92	hypothetical protein	APH_0818	Cytoplasmic	0.4
93	hypothetical protein	APH_0841	Cytoplasmic	0.4
94	hypothetical protein	APH_1382	Cytoplasmic	0.4
95	hypothetical protein	APH_0550	Cytoplasmic	0.4
96	hypothetical protein	APH_0485	Cytoplasmic	0.4
97	hypothetical protein	APH_0355	Inner Membrane	0.4
98	hypothetical protein	APH_1132	Inner Membrane	0.3
99	hypothetical protein	APH_0720	Outer Membrane	0.3
100	hypothetical protein	APH_1384	Outer Membrane	0.3
101	hypothetical protein	APH_1380	Cytoplasmic	0.3
102	hypothetical protein	APH_1370	Cytoplasmic	0.3
103	hypothetical protein	APH_0320	Cytoplasmic	0.3
104	hypothetical protein	APH_0726	Membrane	0.3
105	hypothetical protein	APH_1369	Cytoplasmic	0.3
106	hypothetical protein	APH_1368	Cytoplasmic	0.3
107	hypothetical protein	APH_1385	Cytoplasmic	0.2
108	hypothetical protein	APH_0724	Membrane	0.2
109	hypothetical protein	APH_0805	Outer Membrane	0.2
110	hypothetical protein	APH_0723	Membrane	0.2
111	hypothetical protein	APH_0487	Inner Membrane	0.2
112	hypothetical protein	APH_1386	Cytoplasmic	0.2
113	hypothetical protein	APH_0177	Extracellular	0.1
114	hypothetical protein	APH_0546	Extracellular	0.1
115	major surface protein 4	APH_1240	Outer Membrane	0.1
116	hypothetical protein	APH_0916	Inner Membrane	0.1
117	hypothetical protein	APH_0406	Outer Membrane	0.1
	3/41 named genes ≥ 2-fold up in Ap -ISE6 = 93% hypothetical		19/41 genes membrane associated = 46%

Genes differentially transcribed (p ≤ 0.05, ≥ two-fold difference) between human (HL-60) and tick (ISE6) cells. Gene products (117) are listed in descending order of their transcript abundance in HL-60, with their fold change indicated in the right hand column. Gene products 1–76 were those more highly transcribed in HL-60, and 77–117 were those more highly transcribed in ISE6. Gene products predicted to be membrane associated (in bold) tended to be those most differentially transcribed – at the top (most abundant in Ap-HL-60) and bottom (most abundant in Ap-ISE6) of the list. The percentages of hypothetical genes, and the percentages of gene products that are membrane associated are also indicated. (~25% of all Ap genes code for proteins that are membrane associated.)

Back to article page

ISSN: 1471-2164

Contact us

Submission enquiries: bmcgenomics@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Genomics

Contact us