Figure 5

Expression pattern of the nervy enhancer-GFP reporter transgene during embryonic CNS and PNS development. Shown are GFP immunostains of stage 10 (A) and stage 13 (B) embryos from a transformant line that contains the nervy upstream genomic sequence (shown in Figure 4A) adjacent to a minimal promoter/GFP reporter transgene (anterior is up). A) During early nervous system development, GFP reporter expression is detected in CNS neuroblasts and in PNS sensory organ precursor cells. The letter S indicates the PNS sensory organ precursor column and letters L, I, and M mark the lateral, intermediate and medial CNS neuroblast columns, respectively. Arrow indicates the ventral cord midline. B) GFP reporter expression is also detected in the secondary precursor cells of the developing PNS. Shown is the right half of the thoracic and abdominal segments. The letters V, V', L and D indicate the ventral, lateral and dorsal PNS neuronal cell clusters [59].