Table 4 RT-PCR oligonucleotide primers and conditions.
From: Evidence of the role of tick subolesin in gene expression
Gene description | Upstream/downstream primer sequences (5'-3') | PCR annealing conditions |
|---|---|---|
R. microplus | ||
GI [Genbank:EU436162] | CACCATCACTGAAAGCGGT GTGTTAAATAGTTATGCTCATTAAGAC | 50°C, 30 sec |
GII [Genbank:EU436163] | GATCATTGACCTGGTACCTTCC GACTTGATGACACCGACGG | 50°C, 30 sec |
Subolesin [Genbank:DQ923495] | CACAGTCCGAGTGGCAGAT GATGCACTGGTGACGAGAGA | 50°C, 30 sec |
Beta actin [Genbank:AY255624] | CAC GGT ATC GTC ACC AAC TG TGA TCT GCG TCA TCT TCT CG | 50°C, 30 sec |
I. scapularis | ||
Putative secreted salivary WC peptide [Genbank:AAY66498] | GATATTGATCCAGCCGGAGA ATGTCGTCCCTCCATTGTGT | 60°C, 30 sec |
Putative secreted protein [Genbank:AAM93633] | TGAAGGCAACCATTGCAGTT ATTGATGGCAATCCTGTGGA | 56°C, 30 sec |
Identical to M. rosenbergii Cu-Zn SOD [Genbank:AAZ29240] | TGACCTGGGCAACGTTGA ATGACGCAGCAGGCAATG | 54°C, 30 sec |
Subolesin [Genbank:AY652654] | AGCAGCTCTGCTTCTCGTCT TCGTACTCGTCGCGTATCTG | 54°C, 30 sec |
Beta actin [Genbank:AF426178] | GAGAAGATGACCCAGATCA GTTGCCGATGGTGATCACC | 50°C, 30 sec |