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Table 1 List of spots identified by LC-ESI-MS/MS and bioinformatic analysis. Proteins were classified according to MIPS FunCat. Additional data about mass spectrometry are reported in the additional file 1.

From: Proteome changes in the skin of the grape cultivar Barbera among different stages of ripening

Spot ID Accession number Protein description Name abbreviation Mra Mrb pIa pIb a.a. cov.c(%)
Glycolysis and gluconeogenesis
365 Q9ZSQ4 Cytoplasmic phosphoglucomutase PGluM 68.17 63.12 6.16 5.49 9.6
397 Q42908 2,3-bisphosphoglycerate-independent phosphoglycerate mutase PGlyM-1 63.34 61.18 5.83 5.39 5.7
561 P42896 Enolase ENO-1 52.48 47.91 5.89 5.56 35.5
596 P42896 Enolase ENO-2 52.04 47.91 6.14 5.56 31.5
829 CAN81988 Phosphoglycerate kinase(d) PGK-1 40.66 42.42 6.15 6.29 27.9
863 CAN81988 Phosphoglycerate kinase(d) PGK-2 39.06 42.42 6.21 6.29 37.1
902 ABC75834 Glyceraldehyde-3-phosphate dehydrogenase G3PDH-1 37.48 36.76 7.48 6.72 25.1
937 P26518 Glyceraldehyde-3-phosphate dehydrogenase G3PDH-2 36.77 36.98 7.94 7.09 22.0
1767 Q42908 2,3-bisphosphoglycerate-independent phosphoglycerate mutase PGlyM-2 62.15 61.18 5.78 5.39 16.3
C-compound and carbohydrate metabolism
191 AAC26045 Aconitase-iron regulated protein 1 ACO 102.81 98.09 5.96 5.95 14.2
325 CAN60522 Transketolase(d) TK-1 74.77 73.77 5.97 6.36 15.3
327 CAN60522 Transketolase(d) TK-2 74.34 73.77 6.03 6.36 10.1
378 P51615 NADP-dependent malic enzyme NADP-ME 66.00 65.23 6.10 6.09 20.6
412 AAB47171 Vacuolar invertase 1 GIN1-1 59.07 71.55 4.27 4.60 7.9
413 CAN69570 Putative oxalyl-CoA decarboxylase(d) OxD 60.17 61.06 5.98 5.94 23.8
431 AAB47171 Vacuolar invertase 1 GIN1-2 59.20 71.55 4.33 4.60 8.1
851 P52904 Pyruvate dehydrogenase E1 component subunit β, mitochondrial precursor PDHE1 39.59 38.79 5.17 5.88 7.2
1109 CAN78176 Xyloglucan endotransglycosylase(d) XET 31.65 33.18 6.19 5.98 12.5
Photosynthesis
1088 CAN61828 Manganese-stablising protein/photosystem II polypeptide(d) MnSpPSII 31.91 33.23 5.39 5.87 12.2
Nucleobase metabolism
844 AAU14832 Adenosine kinase isoform 1S ADK 40.04 37.44 5.60 5.07 19.7
Amino acid metabolism
172 CAN63089 Glycine cleavage system P-protein(d) GCPp 109.41 112.81 6.37 6.99 4.9
270 CAN73135 Cobalamin-independent methionine synthase(d) MetSy-1 83.47 81.64 5.97 6.19 11.9
273 CAN73135 Cobalamin-independent methionine synthase(d) MetSy-2 82.38 81.64 5.98 6.19 14.4
572 NP_193129 Serine hydroxymethyltransferase 4 SHM4 52.88 51.72 7.27 6.80 12.3
612 AAO92257 γ-aminobutyrate transaminase subunit precursor isozyme 3 ATpL3 50.98 57.24 6.65 6.72 20.6
654 AAG09278 Ornithine aminotransferase OAT 48.56 51.32 6.21 6.44 9.4
815 P37833 Aspartate aminotransferase cytoplasmic AsAT 41.43 44.51 7.31 7.75 17.7
Transcription
1189 BAF46352 α chain of nascent polypeptide associated complex PAC 28.78 21.92 4.06 4.32 33.7
1511 ABE01085 BTF3 BTF3 17.26 17.34 5.52 6.32 11.9
Protein synthesis
1606 AAL13082 Putative glycine-rich RNA-binding protein GlyRp 13.56 17.33 5.33 7.84 30.3
Protein destination
442 Q43116 Protein disulfide-isomerase precursor PDIpr 58.22 55.56 4.92 4.95 29.7
490 CAN68309 Heat shock chaperonin-binding motif(d) HSC 56.04 41.04 4.94 4.94 17.1
1449 CAN60868 Molecular chaperone(d) MChap-1 19.64 18.23 6.59 6.78 6.9
1513 CAN65631 Molecular chaperone(d) MChap-2 17.26 18.15 5.73 6.17 8.8
1533 P27880 18.2 kDa class I heat shock protein Hsp18.2 16.56 18.17 6.85 5.81 12.0
Cellular communication/signal transduction
1016 CAN81470 Annexin(d) Annex 34.86 35.19 6.92 7.13 29.4
Secondary metabolism
986 CAN60921 Kynurenine formamidase(d) KF 35.46 29.87 5.54 5.15 9.6
1008 CAI56335 Isoflavone reductase-like protein 6 IFRL6 35.23 33.93 6.09 6.02 30.8
1028 CAI56334 Isoflavone reductase-like protein 5 IFRL5 34.38 33.89 6.21 5.76 25.5
Stress
362 NP_001031620 Binding – stress inducible protein(d) BSP 68.17 63.71 6.05 6.00 14.9
521 AAL83720 Catalase CAT 54.44 56.98 7.10 6.71 13.0
810 AAB41022 Polyphenol oxidase PPO-1 41.26 67.39 6.88 6.39 8.4
819 AAB41022 Polyphenol oxidase PPO-2 40.50 67.39 6.64 6.39 15.0
826 AAB41022 Polyphenol oxidase PPO-3 41.09 67.39 6.81 6.39 6.1
843 AAB41022 Polyphenol oxidase PPO-4 39.96 67.39 6.43 6.39 17.5
876 AAB41022 Polyphenol oxidase PPO-5 38.98 67.39 5.99 6.39 9.6
906 CAN78553 Late embryogenesis abundant protein(d) LEA 37.71 34.94 4.43 4.67 22.4
1071 CAB60154 1,3 β glucanase Glucβ-1 32.26 13.37 5.99 6.11 39.3
1075 CAB91554 1,3 β glucanase Glucβ-2 32.65 37.46 6.44 9.45 15.6
1148 AAQ10093 Class IV chitinase Chit4-1 30.19 27.53 4.57 5.38 9.1
1177 AAB65776 Class IV endochitinase EnChit4 28.50 27.24 4.93 5.38 21.1
1226 AAQ10093 Class IV chitinase Chit4-2 27.66 27.53 6.87 5.38 14.4
1240 AAQ10093 Class IV chitinase Chit4-3 26.99 27.53 7.35 5.38 14.4
1316 AAB61590 VVTL1 TLP 24.62 23.97 4.69 5.09 9.0
1318 ABC86744 Abscisic stress ripening protein ASR-1 24.30 16.69 5.81 5.68 30.2
1358 ABC86744 Abscisic stress ripening protein ASR-2 23.94 16.69 5.77 5.68 30.2
1385 ABB02395 Temperature-induced lipocalin TInLi 22.87 21.54 6.42 6.63 13.0
1408 AAQ03092 Glutathione peroxidase GPOX 21.53 18.53 6.52 6.13 23.8
1417 ABC86744 Abscisic stress ripening protein ASR-3 21.22 16.69 5.73 5.68 26.2
1444 CAC16165 Pathogenesis-related protein 10 PR10-1 19.76 17.13 6.11 5.96 22.8
1481 AAB41022 Polyphenol oxidase PPO-6 18.50 67.39 4.91 6.39 5.9
1482 AAB41022 Polyphenol oxidase PPO-7 18.41 67.39 4.99 6.39 10.7
1508 CAN83049 Pathogenesis-related protein Bet v I family(d) PRBetv1 17.20 17.10 5.15 5.12 17.2
1524 ABD78554 Pathogenesis-related protein 10.1 PR10-2 16.75 17.45 6.61 6.07 30.2
1768 AAB41022 Polyphenol oxidase PPO-8 18.45 67.39 4.79 6.39 6.4
Unclassified
476 CAN67811 Dihydrolipoamide dehydrogenase(d) Uncla-1 56.94 49.57 6.13 7.18 9.6
1181 CAN64479 14-3-3 protein(d) Uncla-2 28.29 28.78 4.67 4.78 16.1
1441 ABK64186 CBS domain-containing protein Uncla-3 19.84 22.25 6.95 9.24 25.2
Unknown
1083 NP_001061484 Protein of unknown function DUF52 family(d) Unk 32.53 33.55 6.22 6.11 16.4
  1. a: Experimental molecular weight (kDa) or isoelectric point
  2. b: Theoretical molecular weight (kDa) or isoelectric point.
  3. c: amino acid coverage (%)
  4. d: The protein was reported as a hypothetical protein. In the features, the similarity and function of the identified genes has been annotated by the authors according to Gene Ontology http://www.geneontology.org.