Table 1 List of spots identified by LC-ESI-MS/MS and bioinformatic analysis. Proteins were classified according to MIPS FunCat. Additional data about mass spectrometry are reported in the additional file 1.

365

Q9ZSQ4

Cytoplasmic phosphoglucomutase

PGluM

68.17

63.12

6.16

5.49

9.6

397

Q42908

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

PGlyM-1

63.34

61.18

5.83

5.39

5.7

561

P42896

Enolase

ENO-1

52.48

47.91

5.89

5.56

35.5

596

P42896

Enolase

ENO-2

52.04

47.91

6.14

5.56

31.5

829

CAN81988

Phosphoglycerate kinase^(d)

PGK-1

40.66

42.42

6.15

6.29

27.9

863

CAN81988

Phosphoglycerate kinase^(d)

PGK-2

39.06

42.42

6.21

6.29

37.1

902

ABC75834

Glyceraldehyde-3-phosphate dehydrogenase

G3PDH-1

37.48

36.76

7.48

6.72

25.1

937

P26518

Glyceraldehyde-3-phosphate dehydrogenase

G3PDH-2

36.77

36.98

7.94

7.09

22.0

1767

Q42908

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

PGlyM-2

62.15

61.18

5.78

5.39

16.3

191

AAC26045

Aconitase-iron regulated protein 1

ACO

102.81

98.09

5.96

5.95

14.2

325

CAN60522

Transketolase^(d)

TK-1

74.77

73.77

5.97

6.36

15.3

327

CAN60522

Transketolase^(d)

TK-2

74.34

73.77

6.03

6.36

10.1

378

P51615

NADP-dependent malic enzyme

NADP-ME

66.00

65.23

6.10

6.09

20.6

412

AAB47171

Vacuolar invertase 1

GIN1-1

59.07

71.55

4.27

4.60

7.9

413

CAN69570

Putative oxalyl-CoA decarboxylase^(d)

OxD

60.17

61.06

5.98

5.94

23.8

431

AAB47171

Vacuolar invertase 1

GIN1-2

59.20

71.55

4.33

4.60

8.1

851

P52904

Pyruvate dehydrogenase E1 component subunit β, mitochondrial precursor

PDHE1

39.59

38.79

5.17

5.88

7.2

1109

CAN78176

Xyloglucan endotransglycosylase^(d)

XET

31.65

33.18

6.19

5.98

12.5

1088

CAN61828

Manganese-stablising protein/photosystem II polypeptide^(d)

MnSpPSII

31.91

33.23

5.39

5.87

12.2

844

AAU14832

Adenosine kinase isoform 1S

ADK

40.04

37.44

5.60

5.07

19.7

172

CAN63089

Glycine cleavage system P-protein^(d)

GCPp

109.41

112.81

6.37

6.99

4.9

270

CAN73135

Cobalamin-independent methionine synthase^(d)

MetSy-1

83.47

81.64

5.97

6.19

11.9

273

CAN73135

Cobalamin-independent methionine synthase^(d)

MetSy-2

82.38

81.64

5.98

6.19

14.4

572

NP_193129

Serine hydroxymethyltransferase 4

SHM4

52.88

51.72

7.27

6.80

12.3

612

AAO92257

γ-aminobutyrate transaminase subunit precursor isozyme 3

ATpL3

50.98

57.24

6.65

6.72

20.6

654

AAG09278

Ornithine aminotransferase

OAT

48.56

51.32

6.21

6.44

9.4

815

P37833

Aspartate aminotransferase cytoplasmic

AsAT

41.43

44.51

7.31

7.75

17.7

1189

BAF46352

α chain of nascent polypeptide associated complex

PAC

28.78

21.92

4.06

4.32

33.7

1511

ABE01085

BTF3

BTF3

17.26

17.34

5.52

6.32

11.9

1606

AAL13082

Putative glycine-rich RNA-binding protein

GlyRp

13.56

17.33

5.33

7.84

30.3

442

Q43116

Protein disulfide-isomerase precursor

PDIpr

58.22

55.56

4.92

4.95

29.7

490

CAN68309

Heat shock chaperonin-binding motif^(d)

HSC

56.04

41.04

4.94

4.94

17.1

1449

CAN60868

Molecular chaperone^(d)

MChap-1

19.64

18.23

6.59

6.78

6.9

1513

CAN65631

Molecular chaperone^(d)

MChap-2

17.26

18.15

5.73

6.17

8.8

1533

P27880

18.2 kDa class I heat shock protein

Hsp18.2

16.56

18.17

6.85

5.81

12.0

1016

CAN81470

Annexin^(d)

Annex

34.86

35.19

6.92

7.13

29.4

986

CAN60921

Kynurenine formamidase^(d)

KF

35.46

29.87

5.54

5.15

9.6

1008

CAI56335

Isoflavone reductase-like protein 6

IFRL6

35.23

33.93

6.09

6.02

30.8

1028

CAI56334

Isoflavone reductase-like protein 5

IFRL5

34.38

33.89

6.21

5.76

25.5

362

NP_001031620

Binding – stress inducible protein^(d)

BSP

68.17

63.71

6.05

6.00

14.9

521

AAL83720

Catalase

CAT

54.44

56.98

7.10

6.71

13.0

810

AAB41022

Polyphenol oxidase

PPO-1

41.26

67.39

6.88

6.39

8.4

819

AAB41022

Polyphenol oxidase

PPO-2

40.50

67.39

6.64

6.39

15.0

826

AAB41022

Polyphenol oxidase

PPO-3

41.09

67.39

6.81

6.39

6.1

843

AAB41022

Polyphenol oxidase

PPO-4

39.96

67.39

6.43

6.39

17.5

876

AAB41022

Polyphenol oxidase

PPO-5

38.98

67.39

5.99

6.39

9.6

906

CAN78553

Late embryogenesis abundant protein^(d)

LEA

37.71

34.94

4.43

4.67

22.4

1071

CAB60154

1,3 β glucanase

Glucβ-1

32.26

13.37

5.99

6.11

39.3

1075

CAB91554

1,3 β glucanase

Glucβ-2

32.65

37.46

6.44

9.45

15.6

1148

AAQ10093

Class IV chitinase

Chit4-1

30.19

27.53

4.57

5.38

9.1

1177

AAB65776

Class IV endochitinase

EnChit4

28.50

27.24

4.93

5.38

21.1

1226

AAQ10093

Class IV chitinase

Chit4-2

27.66

27.53

6.87

5.38

14.4

1240

AAQ10093

Class IV chitinase

Chit4-3

26.99

27.53

7.35

5.38

14.4

1316

AAB61590

VVTL1

TLP

24.62

23.97

4.69

5.09

9.0

1318

ABC86744

Abscisic stress ripening protein

ASR-1

24.30

16.69

5.81

5.68

30.2

1358

ABC86744

Abscisic stress ripening protein

ASR-2

23.94

16.69

5.77

5.68

30.2

1385

ABB02395

Temperature-induced lipocalin

TInLi

22.87

21.54

6.42

6.63

13.0

1408

AAQ03092

Glutathione peroxidase

GPOX

21.53

18.53

6.52

6.13

23.8

1417

ABC86744

Abscisic stress ripening protein

ASR-3

21.22

16.69

5.73

5.68

26.2

1444

CAC16165

Pathogenesis-related protein 10

PR10-1

19.76

17.13

6.11

5.96

22.8

1481

AAB41022

Polyphenol oxidase

PPO-6

18.50

67.39

4.91

6.39

5.9

1482

AAB41022

Polyphenol oxidase

PPO-7

18.41

67.39

4.99

6.39

10.7

1508

CAN83049

Pathogenesis-related protein Bet v I family^(d)

PRBetv1

17.20

17.10

5.15

5.12

17.2

1524

ABD78554

Pathogenesis-related protein 10.1

PR10-2

16.75

17.45

6.61

6.07

30.2

1768

AAB41022

Polyphenol oxidase

PPO-8

18.45

67.39

4.79

6.39

6.4

476

CAN67811

Dihydrolipoamide dehydrogenase^(d)

Uncla-1

56.94

49.57

6.13

7.18

9.6

1181

CAN64479

14-3-3 protein^(d)

Uncla-2

28.29

28.78

4.67

4.78

16.1

1441

ABK64186

CBS domain-containing protein

Uncla-3

19.84

22.25

6.95

9.24

25.2

1083

NP_001061484

Protein of unknown function DUF52 family^(d)

Unk

32.53

33.55

6.22

6.11

16.4