BES | Prom. type | ESAG6 type | No. TAR clones | Represent. TAR clone | ESAG5 type | ESAG2 type |
|---|
| Â | Â | DNA | Protein | Â | Â | DNA | Protein | DNA | Protein |
|---|
1
| 1 | 1 | 1 | 18 | A41 | 1 | 1 | 1 | 1 |
2
| 2 | 2a | - | 1 | 2.2–37 | 2 | 2 | -b | - |
3
| 2 | 3 | 1 | 27 | A120 | 2 | 2 | -b | - |
4
| 3 | 4 | 1 | 15 | D18 | 3 | 3 | 2c | 1 |
5
| 4 | 5 | 1 | 17 | B49 | 4 | 3 | 3 | 2 |
6
| 5 | 5 | 1 | 35 | D30 | 4 | 3 | 4 | 3 |
7
| 6 | 6 | 2 | 22 | C10 | 4 | 3 | 5 | 4 |
8
| 7 | 7 | 3 | 8 | D102 | 5 | 4 | 6 | 5 |
9
| 8 | 8 | 4 | 3 | 2.1–9 | 4 | 3 | 7 | 6 |
10
| 8 | 9 | 5 | 24 | B46 | 6 | 5 | 8 | 6 |
11
| 9 | 10 | 6 | 16 | C49 | 4 | 3 | 9 | 7 |
12
| 10 | 11 | 7 | 17 | 98 | 7 | 6 | 10 | 8 |
13
| 11 | 11 | 7 | 1 | A121 | 7 | 6 | 10 | 8 |
Total
|
11
|
11
|
7
|
204
| Â |
7
|
6
|
10
|
8
|
- Overview of T. b. gambiense DAL 972 TAR clone library. A total of 204 T. b. gambiense TAR clones were isolated in yeast and typed into 13 different BES sets after sequencing over the promoter and ESAG6 regions. The different BES promoter and ESAG6 sequence types (either at the DNA or protein level) are indicated with numbers. Two TAR clones were chosen from each BES type (the name of one representative clone is indicated in the chart), and the full length ESAG6, ESAG5 and ESAG2 ORFs were isolated and sequenced from both of these two clones. The sequence types of the ESAG5 and ESAG2 are indicated.
- aPartial sequence.
- bPresence not detected by PCR. See Materials and Methods for diagnostic primers that were used for each ESAG.
- cApproximately 300 bp is single stranded sequence.
