Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

Table 3 T. equiperdum STIB 818 BES TAR clone library.

BES	Prom. type	ESAG6 type		No. TAR clones	Represent. TAR clone	ESAG5 type		ESAG2 type
		DNA	Protein			DNA	Protein	DNA	Protein
1	1	1^a	-	9	10	1^b	-	-^c	-
2	2	2	1	1	3	-^c	-	-^c	-
3	3	3^d	-	11	D27	2	1	1	1
4	4	4^d	-	2	25	3	2	-^c	-
5	5	5^b	-	2	17	-^c	-	2	2
6	6	6	2	8	14	4	3	3	3
7	6	7^e	-	7	D44	5	4	4	4
8	6	8^b	-	3	16	6	5	2	2
9	7	9	3	7	7	7	6	1	1
10	8	9	3	9	1	8	7	1	1
11	9	10	4	10	53	9^b	-	-^c	-
12	10	11	5	8	C23	9^b	-	2	2
13	10	12	6	1	K13	9^b	-	2	2
14	11	13	7	4	G47	6	5	-^c	-
15	11	-^f	-	8	E6	10	8	2	2
16	12^g	-^f	-	1	J46	11	9	-^c	-
Total	12	13	7 ^h	91		11	9	4	4

Overview of T. equiperdum STIB 818 TAR clone library. A total of 91 TAR clones were isolated and typed into 16 different BES sets. The sequence typing was performed as described for Table 1. As done previously, two TAR clones were chosen for each BES type (the name of one clone is indicated in the chart), and the full length ESAG6, ESAG5 and ESAG2 ORFs were isolated and sequenced from both of these two clones.
^aDouble sequence in the centre indicating two sequence types.
^bPseudogene.
^cPresence not detected by PCR. See Materials and Methods for diagnostic primers that were used for each ESAG.
^dPartial sequence.
^eDouble sequence indicating two sequence types within the same BES.
^f Sequence failed.
^gAnti-sense sequence strand only.
^hProbably an underestimate.

ISSN: 1471-2164