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Table 3 T. equiperdum STIB 818 BES TAR clone library.

From: Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

BES Prom. type ESAG6 type No. TAR clones Represent. TAR clone ESAG5 type ESAG2 type
   DNA Protein    DNA Protein DNA Protein
1 1 1a - 9 10 1b - -c -
2 2 2 1 1 3 -c - -c -
3 3 3d - 11 D27 2 1 1 1
4 4 4d - 2 25 3 2 -c -
5 5 5b - 2 17 -c - 2 2
6 6 6 2 8 14 4 3 3 3
7 6 7e - 7 D44 5 4 4 4
8 6 8b - 3 16 6 5 2 2
9 7 9 3 7 7 7 6 1 1
10 8 9 3 9 1 8 7 1 1
11 9 10 4 10 53 9b - -c -
12 10 11 5 8 C23 9b - 2 2
13 10 12 6 1 K13 9b - 2 2
14 11 13 7 4 G47 6 5 -c -
15 11 -f - 8 E6 10 8 2 2
16 12g -f - 1 J46 11 9 -c -
Total 12 13 7 h 91   11 9 4 4
  1. Overview of T. equiperdum STIB 818 TAR clone library. A total of 91 TAR clones were isolated and typed into 16 different BES sets. The sequence typing was performed as described for Table 1. As done previously, two TAR clones were chosen for each BES type (the name of one clone is indicated in the chart), and the full length ESAG6, ESAG5 and ESAG2 ORFs were isolated and sequenced from both of these two clones.
  2. aDouble sequence in the centre indicating two sequence types.
  3. bPseudogene.
  4. cPresence not detected by PCR. See Materials and Methods for diagnostic primers that were used for each ESAG.
  5. dPartial sequence.
  6. eDouble sequence indicating two sequence types within the same BES.
  7. f Sequence failed.
  8. gAnti-sense sequence strand only.
  9. hProbably an underestimate.