Figure 8
From: Gene organization, evolution and expression of the microtubule-associated protein ASAP (MAP9)
Expression of ASAP in human and murine tissues. (A) Northern-blot analysis of ASAP transcript in adult human tissues was carried out with pre-made Northern blots purchased from Clontech. The blots were hybridized with a 32P-labelled full-length ASAP cDNA probe. The sizes of molecular weight marker are shown on the left. (B) RT-PCR analysis of ASAP in murine tissues. Total RNA was prepared from various mouse tissues. A 279-bp ASAP RT-PCR product is visualized. Expression of GAPD was determined as a control of RNA integrity. (C) Western-blot analysis of mASAP protein was carried out using the mASAP antibody on testis, brain, spleen and colon mouse tissue extracts and compared to the profile observed on NIH3T3 and U2-OS (positive controls) or HT29 (negative control derived from colon) cell extracts. α-tubulin levels served as loading control.