Systematic sequencing of mRNA from the Antarctic krill (Euphausia superba) and first tissue specific transcriptional signature

Table 3 Results of EST assembly for each krill cDNA library.

Library	Tissue	Total ESTs	Discarded ESTs	Analyzed ESTs	# GenBank	# EST in cluster	# cluster	# singletons	# consensus	% redundancy	% discovery
K01	Head	35	9	26	ES542703–ES542728	12	10	14	24	38.46	61.54
K05	Head	579	124	455	ES542729–ES543183	225	108	230	338	35.16	64.84
K06	Abdomen	508	47	461	ES543184–ES543644	250	110	211	321	42.73	57.27
K07	Photophores	506	52	454	ES543645–ES544098	255	114	199	313	49.78	50.22
K09	Thoracopods	418	44	374	ES544099–ES544472	227	108	147	255	55.61	44.39
Overall		2046	276	1770		969	450	801	1251	44.35	55.65

Total ESTs = number of produced chromatograms; Discarded ESTs = number of low quality ESTs; Analyzed ESTs = number of sequences processed for clustering; # GenBank = accession numbers of ESTs deposited in GenBank; # EST in cluster = number of sequences in cluster; # clusters = total number of clusters; # singletons = number of putative transcripts identified by one EST; # consensus = number of non-redundant sequences; % redundancy = percentage of sequences not identified in an exclusive manner; % discovery = percentage of ESTs identifying putative new transcripts in the total EST analyzed.

ISSN: 1471-2164