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Table 1 ENU-induced mutation frequencies

From: Improved generation of rat gene knockouts by target-selected mutagenesis in mismatch repair-deficient animals

Genotype/Dosea

Msh6-/-3 × 25

Msh6-/-3 × 30

Msh6-/-3 × 35

Total

WT Control 3 × 40b

# Males injected

6

8

8

24

10

# Fertile males c

N/D

5

1

 

6

# Pups for screening

16

291

3

310

362

# Bases screened

3.6 × 106

69.7 × 106

0.7 × 106

74.0 × 106

37.3 × 106

# Mutations

     

   - nonsense

0

4

0

4

0

   - missense

4

59

1

64

17

   - silent

0

16

0

16

7

   - non-coding

0

5

0

5

6

   - total

4

84

1

89

30

Mutation Frequencies (bp-1)

1.12 × 10-6

1.21 × 10-6

1.38 × 10-6

 

8.05 × 10-7

Mutation Rates (bp)

1 in 8.93 × 105

1 in 8.29 × 105

1 in 7.22 × 105

 

1 in 12.4 × 105

  1. aDose is indicated as mg of ENU per kg bodyweight (three weekly injections)
  2. bWT (Wild Type) control 3 × 40 mg/kg measurements were adapted from [5] and represent the highest ENU-induced mutation frequency in rats
  3. cFertility is measured by the presence of at least one litter within 10 weeks after the last injection.
  4. Sterility was confirmed by histological examination (N/D = not determined)
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BMC Genomics

ISSN: 1471-2164

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