Figure 1
Type and location of modified nucleosides in tRNAs of H. volcanii . (A) 2D-Cloverleaf representation of tRNA. Group of nucleosides in boxes are present at the same location but in different isoacceptors species. Modified nucleosides in white and in a gray background are uniquely present at that position in archaeal tRNAs so far. Those indicated in black are also found in tRNAs from Bacteria and/or Eukarya. See text for references on Abbreviations. The large gray box including the m1Ψ containing branch and the G+ containing branch encompass the interacting parts of the tRNA molecule that forms the 3D-core. (B) Schematic representation of tertiary interactions in tRNA structure. Each nucleoside involved in stacking or base pairing with another nucleotide within the 3D-core (gray background box) is represented by a rectangle. Other parts of the tRNA (anticodon branch and amino acid stem are represented by lines. Inside the large gray rectangle are the elements that contribute to the 3D interaction, allowing an L-shaped spatial conformation to be formed from the 2D cloverleaf structure.