Table 3 Primers used for amplification of large fragments in Crassostrea. hongkongensis mitochondrial genome
Order | Primer name | Sequence (5'-3') | Amplification conditions* | Product size (bp) |
|---|---|---|---|---|
1 | RCOIA Mt133 | GGTCAACAAATCATAAAGATATTGG CCGGTCTGAACTCAAATCA | 35× (94°C 1 min, 57°C for 1.5 min, 72°C for 1.5 min) | 2325 |
2 | Mt89 1R | CAGTACCTGCCCAGTGCGACAT GGCTTAATTACGGCTGGTGTTT | 35× (94°C 1 min, 57.5°C for 1.5 min, 72°C for .5 min) | 2626 |
3 | CYF 12-B | TTAGAGTTCCGTTTCACCCG CTTTCGCTGCGGTTTAGTTAGT | 35× (94°C 1 min, 47.5°C for 1 min, 72°C for 1.5 min) | 2448 |
4 | 12-F 12B | GGTTCTGGTCTAATGTTCGCT GTTACTCTCCCTTTACTCCC | 35× (94°C 1 min, 47°C for 1 min, 72°C for 1 min) | 804 |
5 | 12RF ATP-H | GTAGGTCAGGACGAAGTGCT AGAGCACAGGTGTTGGGAGA | 35× (94°C 1 min, 49°C for 1 min, 72°C for 1 min) | 996 |
6 | 727F DNR | TATTCGCCCTGACACTCTTAC TCCCGTATTCCAGAAGAAGCAG | 35× (94°C 1 min, 77.5°C for 1 min, 72°C for 1.5 min) | 2444 |
7 | ND2F 5NR | AGATTTGCTGGTTTTCCTCCG CTGATGTTGTAAGTCCCGCAC | 35× (94°C 1 min, 49.5°C for 1 min, 72°C for 1.5 min) | 2641 |
8 | ND6F2 NDR | GCTCCTACTCCTGTCTCATC TGACGCTTGCGAATAAGACAC | 35× (94°C 1 min, 49°C for 1 min, 72°C for 1 min) | 948 |
9 | ND5F RND | GAGGATTTACTGCGGTGATTGG GTCAGAGCCATTCCGACTATC | 35× (94°C 1 min, 47.5°C for 1 min, 72°C for 1.5 min) | 1373 |
10 | FLeu NO1 | GCCAGGTTAGTGTGGTATTTAG GAGTAAGTGGATAAGGGTGGA | 35× (94°C 1 min, 47.5°C for 1 min, 72°C for 1.5 min) | 2017 |