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Table 2 Restriction enzymes used for simulations

From: Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA

  Restriction enzyme Recognition site Occurrence in RefSeq EST sets (%)
    Arabidopsis thaliana Homo sapiens Mus musculus
1 MboI GATC 97.2 98.8 82.5
2 HpaII CCGG 84.4 93.3 64.7
3 HinP1I GCGC 60.3 89.4 61.7
4 Csp6I GTAC 87.9 97.5 81.4
5 TaqI TCGA 95.3 89.4 64.2
6 TasI AATT 97.5 98.2 80.2
7 MseI TTAA 96.3 97.2 78.9
8 FatI CATG 96.4 99.7 83.5
9 MaeI CTAG 88.6 95.1 76.3
10 MaeII ACGT 86.7 92.0 64.8
11 ApoI RAATTY 82.7 86.2 68.5
12 BstYI RGATCY 80.0 90.5 70.4
13 AcyI GRCGYC 37.8 70.3 46.4
14 BmeT110I CYCGRG 49.6 79.3 52.8
15 Cfr10I RCCGGY 49.6 68.6 45.1
16 CfrI YGGCCR 45.3 90.3 68.7
17 BsaWI WCCGGW 64.1 60.1 44.2
18 TatI WGTACW 63.5 86.8 72.7
  1. All these restriction enzymes are commercially available, supplying companies can be found on the REBASE [23] homepage. Frequency of occurrence in EST sets were calculated using NCBI Reference Sequence collections without repetition, sequences containing more than one copy of a recognition site counted only once.