Copy number and genomic distribution of PwRn1. (A) Reverse dot-blot analysis. The membrane was dotted in duplicate with varying amounts of Pw-D-13 fragment as shown on the top and probed with the genomic DNA of diploids. The blots of cysteine protease (Cys Prot) were used as a single copy control. The signal intensities were compared to estimate the copy number of PwRn1. (B) Southern blotting of PwRn1 with the genomic DNAs of diploid (Haenam, Hn) and triploid (Bogil-do, Bg) Paragonimus westermani. Restriction endonucleases used for the digestion of DNAs are presented at the top. The positions of DNA size standards (in kb) are shown on the left.