The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules

Table 1 Relative expression levels of genes belonging to the regulons of McbR, SsuR, and/or CysR measured by real-time RT-PCR

CDS	Name	Regulon^a	Function	mRNA ratio WT/CR030	comparing^b CR031/CR032
Cg0012	ssuR	McbR, CysR	Transcriptional activator of sulphonate(ester) utilisation	32.9	12.8
Cg0156	cysR	McbR	Transcriptional activator of assimilatory sulphate reduction	0.61	0.76
Cg0755	metY	McbR	O-acetylhomoserine sulfhydrylase	1.5	1.0
Cg1514		CysR*	Secreted protein of unknown function	137.9	158.1
Cg1376	ssuD1	McbR, SsuR	FMNH₂-dependent aliphatic sulphonate monooxygenase	186.8	1.3
Cg2810		McbR, CysR	Putative secondary H⁺/Na⁺ symporter	0.39	0.14
Cg3118	cysI	McbR, CysR	Ferredoxin-sulphite reductase	104.7	93.4
Cg3119	fpr2	McbR, CysR	Ferredoxin-NADP⁺ reductase	21.6	8.8
Cg3138		CysR*	Putative integral membrane protein	0.0078	0.018
Cg3253	mcbR	McbR	Transcriptional repressor of sulphur metabolism	0.97	0.88
Cg3372		McbR, CysR	Conserved protein of unknown function	99.0	22.9

^a According to the studies of [7, 15], and this study.
^b Total RNA was isolated from C. glutamicum cultures grown in minimal medium with 2 mM L-cysteine as sole sulphur source to o.D.₆₀₀ 10 and then subjected to sulphur starvation for 30 min. Relative expression levels were measured for two independent replicates with at least three technical replicates each.
* predicted from microarray data and motif search.

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