A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)

Table 2 Spruce EST summary

Total sequences	206,875
Number of 5' sequences	41,472
Number of 3' sequences	165,403
Average assembled 3' EST length (bp)^a	656.4
Number of high-quality 3' sequences^b	147,146
Number of contigs^c	19,941
Number of singletons	26,804
Number of putative unique transcripts^d	46,745
Number of assembled 3' ESTs with^e
Significant BLASTX match	96,454
No significant BLASTX match	50,692
Average number of contig members	6.03
Number of contigs containing
2 ESTs	6,050
3–5 ESTs	7,449
6–10 ESTs	3,841
11–20 ESTs	1,941
21–50 ESTs	572
>50 ESTs	88

^aHigh-quality (hq) sequences only.
^bA sequence is considered of hq if it is not derived from contaminant species and its vector-trimmed and poor-quality-trimmed PHRED 20 length is >100 bases.
^cA contig (contiguous sequence) contains two or more ESTs; 3' sequences only.
^dNumber of putative unique transcripts (PUTs) among assembled 3' ESTs equals the number of contigs plus the number of singletons.
^eThreshold for BLASTX significance versus the non-redundant (NR) database of GenBank is a score value > 50.

ISSN: 1471-2164