Figure 2

Individual effect of standard oligonucleotide filters on resolving power. The negative (or absolute value) of the slope in the resolving power curve is shown for individual constraints on oligonucleotides when detecting one-copy deletions with 50-mer probes. For each filter, the green bars correspond to resolving power calculations performed exclusively with oligonucleotides accepted by the filter while for the red bars only the oligonucleotides rejected by the filter were used in the calculations. Solid colours are associated with C. elegans and hashed areas are associated with the human data set. The filters from left to right are: elimination of non-unique 20 mers, elimination of homopolymers longer than 5 nucleotides, the selection of a 10°C range in melting temperature, the elimination of oligonucleotides with self-folding energy smaller than -1 kcal/mol, the elimination of oligonucleotides mapping to more than one genomic region with more than 70% similarity, the elimination of oligonucleotides with 15-mer count above the median value, and finally, the simultaneous application of all the those filters. More details on those standard filters can be found in the Methods section.