Table 1 Real-time qPCR analyses
Target | Primer sequence from 5' to 3' | Amplicon size (bp) | Accesion number |
|---|---|---|---|
Matrix metalloproteinase 13 | F CCAAAAAGAGGGCACCAGATGG | 53 | DW539943 |
| Â | R CCAAAAAGAGGGCACCAGATGG | Â | Â |
Matrix metalloproteinase 9 | F AGTCTACGGTAGCAGCAATGAAGGC | 72 | CA342769 |
| Â | R CGTCAAAGGTCTGGTAGGAGCGTAT | Â | Â |
Cathepsin S | F CGAAGGGAGGTCTGGGAGAGGAAT | 87 | CA355014 |
| Â | R GCCCAGGTCATAGGTGTGCATGTC | Â | Â |
Bone morphogenetic protein 4 | F TCAAGTTGCCCATAGTCAGT | 207 | CA056395 |
| Â | R CACCTGAACTCTACCAACCA | Â | Â |
Alkaline phosphatase | F CTAGTTTGGGTCGTGGTATGT | 185 | CA358635 |
| Â | R TGAGGGCATTCTTCAAAGTA | Â | Â |
Heat shock protein 90β-20 | F GAACCTCTGCAAGCTCATGAAGGA | 72 | CF752846 |
| Â | R ACCAGCCTGTTTGACACAGTCACCT | Â | Â |
Collagen 10α | F TGGTGCTCTTTGACTGCCTGTAA | 180 | EG837148 |
| Â | R CATCCTGTGTGTTGCAATATCACA | Â | Â |
Collagen 1α | F AGAGAGGAGTCATGGGACCCGTT | 155 |  |
| Â | R GGGTCCTGGAAGTCCCTGGAAT | Â | Â |
Collagen 2α | F TGGTCGTTCTGGAGAGACT | 151 | BX865386 |
| Â | R CCTCATGTACCTCAAGGGAT | Â | Â |
Decorin | F GAACCTGGCTAAGCTGGGTCTAA | 256 | DQ452069 |
| Â | R GAACAGGCTGATGCCAGAGTACAT | Â | Â |
Elastin | F GAGGCTACAGACCAGGAGGAGTT | 226 | BU694149 |
| Â | R TCTGGGTCGGTGGGTTTGTA | Â | Â |
Laminin | F CATGTGACATGGACACAGGAA | 273 | DY722974 |
| Â | R CGTCCTCAGCCTCATAGGTGTA | Â | Â |
CCAAT/enhancer binding protein β | F TACGTCCTGGGCTATCCTGAACTGC | 140 | CA348284 |
| Â | R CCAGACGAACCGTTGTTGTCCA | Â | Â |
Erythroid 5-aminolevulinate synthas | F CACATGAGACAGCTGCTCCTGGAGA | 121 | DW580939 |
| Â | R GCTCCAGCAAGATGTCACACACCT | Â | Â |
Heme oxygenase 1 | F AGTCAGTGGAGAGAGACCTGGAGCA | 117 | CA363120 |
| Â | R GGTTGTCTTTGCCGATCTGTCTGAG | Â | Â |
Haemoglobin beta chain | F ACAAACGTCAACATGGTCGACTGG | 67 | NM_001123666 |
| Â | R TCTTTCCCCACAGGCCTACGAT | Â | Â |
Mannose binding lectin 1 | F TCCATTGCACTGGGCGATGC | 105 | CA349943 |
| Â | R CACTGCTTCCACCTGAGCCTCA | Â | Â |
Prostaglandin D synthase | F CCTACACCAACCTGAACGCTGATG | 98 | CA352578 |
| Â | R ACGCTGGCTGGTGAAGGTGAAG | Â | Â |
MHC class II α chain | F AGTCAGGTGGACCAGGAACAATCA | 96 | CA379977 |
| Â | R CTGGAGAACTGGTTGAGGGTGAAA | Â | Â |
CD8α | F CGTCTACAGCTGTGCATCAATCAA | 266 | AY693391 |
| Â | R GGCTGTGGTCATTGGTGTAGTC | Â | Â |
IL-12β | F TCTACCTACACGACATTGTCCAGCC | 62 | AJ548830 |
| Â | R ATCCATCACCTGGCACTTCATCC | Â | Â |
Arginase 1 | F AGCCATGCGTATCAGCCAA | 122 | EG929369 |
| Â | AAGGCGATCCACCTCAGTCA | Â | Â |
Programmed death ligand 1 | F TCAACGACTCTGGGGTGTACCGATG | 133 | CA366631 |
| Â | R TCCACCTCATCTCCACCACGTCTC | Â | Â |
Beta-2-microglobulin | F TCGTTGTACTTGTGCTCATTTACAGC | 107 | AF180478 |
| Â | R CAGGGTATTCTTATCTCCAAAGTTGC | Â | Â |
TGF-β | F AATCGGAGAGTTGCTGTGTGCGA | 332 | EU082211+ |
| Â | R GGGTTGTGGTGCTTATACAGAGCCA | Â | AJ007836 |
IL-1 receptor type 1 | F CCAAAAAGAGGGCACCAGATGG | 126 | NM_001123633 |
| Â | R CGTATCGTCTCTCCAACACCTCAGG | Â | Â |
CD4 | F TGCATTGTTCCTCTCTTCCACAGC | 128 | EG852912 |
| Â | R CCGTCCCAAGGTACCATAGTACCAA | Â | Â |
IL-10 | F ATGAGGCTAATGACGAGCTGGAGA | 54 | EF165028 |
| Â | R GGTGTAGAATGCCTTCGTCCAACA | Â | Â |
Eukaryotic translation initiation factor 3 subunit 6 | F GTCGCCGTACCAGCAGGTGATT | 92 | CX040383 |
| Â | R CGTGGGCCATCTTCTTCTCGA | Â | Â |