Tissue expression of rainbow trout zar1 , mos , btg3 and gdf9. Tissue expression of rainbow trout zar1, mos, btg3 and gdf9 transcripts. Real-time PCR analysis was conducted using total RNA originating from the following tissues sampled in 3 different fish: brain, pituitary, gills, heart, liver, stomach, intestine, spleen, head kidney, trunk kidney, muscle, skin, ovary, unfertilized eggs (i.e. metaphase II oocytes), and stage II testis. For each tissue, 3 separate reverse transcription reactions were carried out using separate RNA samples originating from 3 different fish. Reverse transcription reactions were pooled and use to run real-time PCR in triplicates. Mean and standard deviation are shown (N = 3). Expression levels not significantly different from background signal at p < 0.05 are indicated (#). Expression levels are expressed as a percentage of the expression in the ovary. In situ hybridization of rainbow trout ovarian tissue sections in the presence of antisense (AS) probe. Labels: nu = nucleus, oo = ooplasm. Bars represent 200 μm. A smaller view of an adjacent section hybridized with the sense probe is shown for each gene.