ROC curves obtained from different search modes. ROC curves for three different search modes employed when running RAId_DbS using the Aurum dataset composed of 9977 spectra. In panel (A) curves are shown in sensitivity versus (1-specificity), while in panel (B) the cumulative number of true positives versus the cumulative number of false positives are shown. In panel (B), the increase in the number of false positives coming from annotated SAPs/PTMs (red curve) and with novel SAPs (blue curve) is anticipated due to a larger search space compared to searches done within the standard database only. The larger total number of false positives found in the latter methods, however, will push the ROC curves leftwards upon normalizing to 1-specificity.