Figure 4

Genes we identified that are expressed in the DE. (A) Cldn4 is expressed in the anterior DE and in extra-embryonic regions at E7.25, (B) in the foregut pocket, lateral DE, and 37 extra-embryonic regions at E7.75, (C-D) and in the gut endoderm and otic vesicle from E8.5–9.0. (E) Itga3 is strongly expressed throughout the gut endoderm at E8.5. (F) Cpm is expressed in the anterior DE at E7.5, (G) strongly in the foregut pocket and throughout the DE at E7.75, (H) and in the ventral aspect of the gut at E8.5 (Note: at longer exposure, Cpm can be seen in more DE cells; Additional File 3). (I) Ppp1r14a is expressed in the ventral aspect of the gut at E8.5. (J) Efhd2 is highly expressed in the rostral foregut and caudal hindgut at E8.5. (A, F) lateral view, anterior left; (B, G) anterior view; (C-E) lateral view; (H-J) ventral view.