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Table 2 Transcripts where 97% of the variance in gene expression is explained by growth conditions

From: mtDNA depletion confers specific gene expression profiles in human cells grown in culture and in xenograft

 

Affymetrix Probe IDa

Gene Symbol

Entrez GeneID

Gene Description

Growth Prop. of Varianceb

F testc

ρ0 Vivo/ρ0 Vitro

WT Vivo/WT Vivo

       

FCd

P e

FCd

P e

Up-regulated and dependent on growth conditions

211571_s_at

CSPG2

1462

chondroitin sulfate proteoglycan 2

0.99

1.41 × 10-12

34.6

0.0001

19.0

0.0002

 

215646_s_at

CSPG2

1462

chondroitin sulfate proteoglycan 2

0.99

2.52 × 10-12

33.5

0.0003

20.3

0.0003

 

220952_s_at

PLEKHA5

54477

PI 3-phosphate-binding protein-2

0.99

1.79 × 10-11

6.7

0.0003

5.6

0.0006

 

209090_s_at

SH3GLB1

51100

SH3-domain GRB2-like endophilin B1

0.99

2.86 × 10-10

3.0

0.0015

3.1

0.0002

 

216405_at

LGALS1

3956

lectin, galactoside-binding, soluble, 1

0.98

6.43 × 10-12

11.5

0.0007

14.4

0.0006

 

212254_s_at

DST

667

dystonin

0.98

3.64 × 10-11

3.9

0.0006

4.7

0.0003

 

201056_at

GOLGB1

2804

golgin B1, golgi integral mem. protein

0.98

2.12 × 10-10

3.3

0.0008

2.9

0.0004

 

221829_s_at

TNPO1

3842

transportin 1

0.98

2.86 × 10-10

4.0

0.0012

3.9

0.0005

 

204619_s_at

CSPG2

1462

chondroitin sulfate proteoglycan 2

0.98

3.01 × 10-11

12.2

0.0003

7.5

0.0003

 

205173_x_at

CD58

965

CD58 molecule

0.98

1.70 × 10-10

3.5

0.0014

3.7

0.0004

 

218901_at

PLSCR4

57088

phospholipid scramblase 4

0.98

1.32 × 10-9

3.5

0.0009

3.8

0.0014

 

213229_at

DICER1

23405

Dicer1, Dcr-1 homolog

0.97

2.18 × 10-10

6.1

0.0009

7.0

0.0013

 

208772_at

ANKHD1

404734

ankyrin repeat & KH domain containing 1

0.97

1.97 × 10-9

2.5

0.0027

2.4

0.0003

 

221731_x_at

CSPG2

1462

chondroitin sulfate proteoglycan 2

0.97

4.35 × 10-11

104.7

0.0001

30.9

0.0002

 

201057_s_at

GOLGB1

2804

golgin B1, golgi integral mem. protein

0.97

1.39 × 10-9

3.2

0.0027

2.9

0.0002

 

214295_at

KIAA0485

57235

KIAA0485 protein

0.97

4.14 × 10-10

3.9

0.0014

4.1

0.0008

 

201024_x_at

EIF5B

9669

eukaryotic translation initiation factor 5B

0.97

2.66 × 10-9

2.3

0.0012

2.2

0.0012

 

201280_s_at

DAB2

1601

disabled homolog 2

0.97

4.14 × 10-10

5.3

0.0004

3.5

0.0006

 

218396_at

VPS13C

54832

vacuolar protein sorting 13 homolog C

0.97

8.54 × 10-10

4.4

0.0012

4.8

0.0006

 

205383_s_at

ZBTB20

26137

zinc finger and BTB domain containing 20

0.97

5.52 × 10-10

4.2

0.0011

6.7

0.0003

 

219221_at

ZBTB38

253461

zinc finger and BTB domain containing 38

0.97

2.98 × 10-12

6.0

0.0007

6.5

0.0003

 

208663_s_at

TTC3

7267

tetratricopeptide repeat domain 3

0.97

1.19 × 10-9

3.1

0.0021

3.1

0.0013

 

212070_at

GPR56

9289

G protein-coupled receptor 56

0.97

3.78 × 10-10

3.3

0.0007

4.3

0.0008

 

203216_s_at

MYO6

4646

myosin VI

0.97

2.35 × 10-9

3.1

0.0018

2.7

0.0012

 

204620_s_at

CSPG2

1462

chondroitin sulfate proteoglycan 2

0.97

6.97 × 10-11

104.5

0.0001

27.8

0.0002

 

212062_at

ATP9A

10079

ATPase, Class II, type 9A

0.97

2.73 × 10-9

2.5

0.0002

2.7

0.0030

 

213775_x_at

ZNF638

27332

zinc finger protein 638

0.97

2.86 × 10-10

2.6

0.0008

3.0

0.0005

Down-regulated and dependent on growth conditions

213201_s_at

TNNT1

7138

troponin T type 1

0.99

6.43 × 10-12

-6.9

0.0002

-5.0

0.0003

 

212432_at

GRPEL1

80273

GrpE-like 1, mitochondrial

0.99

1.37 × 10-9

-2.3

0.0008

-2.3

0.0005

 

209507_at

RPA3

6119

replication protein A3, 14 kDa

0.98

6.76 × 10-10

-2.2

0.0010

-2.3

0.0002

 

209825_s_at

UCK2

7371

uridine-cytidine kinase 2

0.98

1.09 × 10-9

-2.5

0.0019

-2.3

0.0006

 

202533_s_at

DHFR

1719

dihydrofolate reductase

0.98

3.51 × 10-9

-2.1

0.0013

-2.0

0.0006

 

201577_at

NME1

4830

non-metastatic cells 1, protein expressed

0.97

2.29 × 10-9

-2.1

0.0014

-2.0

0.0004

 

208756_at

EIF3S2

8668

euk. transl. init. factor 3, subunit 2 beta

0.97

1.92 × 10-8

-1.8

0.0002

-1.7

0.0013

 

207239_s_at

PCTK1

5127

PCTAIRE protein kinase 1

0.97

6.29 × 10-9

-1.9

0.0017

-1.9

0.0007

 

204126_s_at

CDC45L

8318

CDC45 cell division cycle 45-like

0.97

2.31 × 10-8

-2.0

0.0030

-1.9

0.0004

 

219162_s_at

MRPL11*

65003

mitochondrial ribosomal protein L11

0.97

1.37 × 10-9

-2.5

0.0010

-2.2

0.0006

 

208847_s_at

ADH5

128

alcohol dehydrogenase 5, chi polypeptide

0.97

5.92 × 10-8

-1.7

0.0022

-1.6

0.0007

 

210250_x_at

ADSL

158

adenylosuccinate lyase

0.97

4.04 × 10-9

-2.0

0.0015

-2.3

0.0012

 

208799_at

PSMB5

5693

proteasome beta 5 subunit

0.97

2.6 × 10-8

-1.9

0.0025

-1.8

0.0004

 

221620_s_at

NOMO3

408050

NODAL modulator 3

0.97

1.64 × 10-9

-2.0

0.0015

-2.3

0.0004

 

210519_s_at

NQO1

1728

NAD(P)H dehydrogenase, quinone 1

0.97

7.60 × 10-9

-2.1

0.0020

-1.8

0.0007

 

208910_s_at

C1QBP

708

splicing factor SF2-associated protein

0.97

1.63 × 10-9

-2.4

0.0020

-2.5

0.0008

 

201903_at

UQCRC1

7384

ubiquinol-cytochrome c reductase

0.97

9.25 × 10-9

-1.8

0.0019

-2.1

0.0009

 

200039_s_at

PSMB2

5690

proteasome subunit, beta type, 2

0.97

5.72 × 10-9

-1.9

0.0022

-2.0

0.0006

 

217960_s_at

TOMM22

56993

mitochondrial import receptor Tom22

0.97

1.04 × 10-8

-2.2

0.0007

-1.9

0.0014

 

205691_at

SYNGR3

9143

synaptogyrin 3

0.97

1.83 × 10-9

-3.1

0.0012

-2.4

0.0012

  1. aOnly probe tilings representing genes with annotated functions are provided
  2. bProportion of variance in gene expression due to growth conditions (i.e. cultured cells or xenografts)
  3. cBayes modified F test (multiple hypothesis corrected)
  4. dFold-change eBenjamini and Hochberg corrected two-tailed Student's t-test