Figure 1

Cercospora zeae-maydis grown on V8 agar for sporulation libraries. For library construction, cultures initiated from conidial suspensions were grown in constant darkness and RNA was extracted (A) three days and (B) five days after initiation, time points that correspond to early and late stages of conidiation, respectively. Conidiophores (red arrows) typically form two to three days after inoculation (C) and conidia (blue arrows) develop and mature over the next 48 hours (D). In contrast, cultures grown on the same medium in constant light produce few conidiophores or conidia (E).