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Table 1 EST libraries constructed for this study

From: Analyses of expressed sequence tags from the maize foliar pathogen Cercospora zeae-maydis identify novel genes expressed during vegetative, infectious, and reproductive growth

Growth Conditions

Library Name

Insert size selection

Unique clones

Total unique ESTs

Insertless clones

Failed quality1

Passing to cluster

Small insert (100–700 bp)

Total number with BLAST hits2

Average diversity (passing clones)

Vegetative

CBYB

2–8 kb

4,992

9,888

2082

806

7000

789

299

58.8%

 

CBYC

0.6–2 kb

1536

3071

164

523

2384

194

260

72.6%

Sporulation

CBYF

2–8 kb

1536

3072

1314

173

1585

305

123

82.3%

 

CBYG

0.6–2 kb

1152

2304

834

186

1284

335

139

76.8%

 

CCAW

None

4608

9216

340

1327

7549

3291

744

43.4%

  1. 1 Comprised of ESTs of low quality sequence, low complexity, and presumptive contaminants.
  2. 2 Evaluated with BLASTn against the non-redundant database