Figure 6

Activation of select transcription factors after partial hepatectomy. Transcription factor activation was monitored at different time points after PHx using TransAm NFκB p65, HNF-1, STAT-3, CREB, GATA family and C/EBP α/β kits (Active Motif, Carlsbad, CA) or TransFactor Universal Colorimetric Kit (Clontech, Mountain View, CA). In each case, 20–50 μg of nuclear extract was added per well with immobilized oligonucleotides based on the corresponding transcription factor binding site sequence. The primary antibodies were used to detect transcription factors bound to their target DNA. Addition of HRP-conjugated secondary antibody provided a colorimetric readout for quantification by spectrophotometry. At each time point, the data is normalized against a blank control sample. Error bars are based on replicate data from three animals.