Table 1 Primers used in QRT-PCR
Gene Name | Symbol | Â | Primer Sequence |
|---|---|---|---|
Actin, beta | Actb | Forward Reverse | 5'-TCGCTGACAGGATGCAGAAG-3' 5'-ACATCTGCTGGAAGGTGGACA-3' |
Annexin A5 | Anxa5 | Forward Reverse | 5'-CTGCCTACCTTGCAGAGACC-3' 5'-CGTGGCGAAGTTCTTCCTAA-3' |
B-cell translocation gene 1, anti-proliferative | Btg1 | Forward Reverse | 5'-GAGGATGGCTCCATCTGTGT-3' 5'-TTTTGGAAGGGCTTGTTCTG-3' |
B-cell translocation gene 2 | Btg2 | Forward Reverse | 5'-GCTCTGTGGTTCTGCCATTTC-3' 5'-CAAAGCTGTGAATCGCTCCAG-3' |
Cathepsin L | CtsL | Forward Reverse | 5'-CAAAGACCGGAACAACCACT-3' 5'-CACTCAGAGACGGGTTTGGT-3' |
CCAAT/enhancer binding protein (C/EBP), beta | Cebpb | Forward Reverse | 5'-GGGTTGTTGCTGTTGATGTTT-3' 5'-CGAAACGGAAAAGGTTCTCA-3' |
Endothelial differentiation G-protein-coupled receptor, 2 | Edg2 | Forward Reverse | 5'-CCATGAACGAACAACAGTGC-3' 5'-AGCATGATGAACACGCAGAC-3' |
FXYD domain-containing ion transport regulator 1 | Fxyd1 | Forward Reverse | 5'-GCAGGAACCAGATCCATTCA-3' 5'-CCCAGTTCTCTGCTGTTGGT-3' |
G0/G1 switch gene 2 | G0s2 | Forward Reverse | 5'-CCCAGAGCTCAGATGGAAAG-3' 5'-ACTAGACCGAGCACCACACC-3' |
General transcription factor 2i | Gtf2i | Forward Reverse | 5'-GGGATGGCTAGCAAAATCAA-3' 5'-CGAACGGTAGAGGTCTGAGG-3' |
Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | Forward Reverse | 5'-AGTTCAACGGCACAGTCAAG-3' 5'-GTGGTGAAGACGCCAGTAGA-3' |
Heme oxygenase 2 | Hmox2 | Forward Reverse | 5'-TCAGTTTTCCAGGCCTTTTG-3' 5'-TTAGAGTGCTGTGGCAGGTG-3' |
Hepatocyte growth factor activator | Hgfac | Forward Reverse | 5'-TGAGTCGACCTCAACTGCAC-3' 5'-AGCCGTTCCCAATGTAGATG-3' |
Insulin-like growth factor binding protein 1 | Igfbp1 | Forward Reverse | 5'-GCCAGGGAGCCTGTGTACTA-3' 5'-AGCAGCTGTTCCTCTGTCA-3' |
Mitogen activated protein kinase 1 | Mapk1 | Forward Reverse | 5'-CCTACGGCATGGTTTGTTCT-3' 5'-TCTCATGTCTGAAGCGCAGT-3' |
Pre-B-cell colony enhancing factor 1 | Pbef1 | Forward Reverse | 5'-CTGTTCCTGAGGGCTCTGTC-3' 5'-TTGTGGCCACTGTAATTGGA-3' |
Superoxide dismutase 2, mitochondrial | Sod2 | Forward Reverse | 5'-TCTGTGGGAGTCCAAGGTTC-3' 5'-ACACATCAATCCCCAGCAGT-3' |
Tubulin, alpha 4 | Tuba4 | Forward Reverse | 5'-AGGAGATCATCGACCCAGTG-3' 5'-ACAGAAAGCCGTTCCATCAG-3' |