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Table 2 Markers developed and cloning and sequencing primers for mapping

From: Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

Gene Primer sequence1 Tm °C2 Cycles3 Allele length (nt)4
Pru du 1.01 For-ATGGGTGTCTTCACATATGAGAG
Rev-CTGACAACATATATACTGTAAC C
58 35 T-218
Pru p 1.01 For-TCATACAGCTACACCTTGT T
Rev-CAATGAGCTTGAAGAGATTTG
58 35 E-205
Pru p/du 1.02 For-GTTGGAACCATCAAGAAGGAC
GTTT CATGAGCATACAGAACTATC
58 35 T-188
E-179
Pru p/du 1.03 For-GGTGAAGGTTAGCTAGTTGA
Rev-GTTT CCATCCCTGTGCTTCACAA
58 35 T-153
E-150
Pru p/du 1.04 For-CTTTGGTGAAGGTAAGTTTAGA
Rev-GTTT CATACCTGTAAGTGCTACCTGC
58 35 T-157
E-164
Pru p/du 1.05 For-GCTTCCAACATCAAGTAATACCT
Rev-G TTTGGGACTAGGTTGTCAGCA
60 35 T-179
E-182
Pru p/du 1.06A For-TAGTTATGAGTTGCTTGCAATGCT
Rev-GAAAGTTCCAAAGTACATGTGC
58 35 1871, sequencing
Pru du 1.06B For-ATCCCCAAGATTGATCCCCT G
Rev-GATTAGAATTTAAGAGCTTACTGT
60/58 5/35 T-182
Pru p 1.06B For-AAAGCCCTTGTTCTTGAAGCG
Rev-GATTAGAATTTTAGAGCTTACTC C
60/58 5/35 E-212
Pru p/du 1.06C For-CTAATTAATAACATAAATCTTGAAAC T
Rev-TTGATCTCAACATCCCCTGC
56/54 5/35 E-272
Pru du 2.01A For-CTTAGCATTCAACCAGCCT C
Rev-GATGAGGTAGTCAGGGCA AG
60 35 T-175
Pru p 2.01A For-CAATTGTCTCTGTAATCTGTTT
Rev-ACGGAGCGTCCACAGAGTT
60 35 E-205
Pru du 2.01B For-TTGCCCTGCCAATGTTAACGC
Rev-GCACTGGGTCTTAAAGAACT G
60 35 T-187
Pru p 2.01B For-CAATTGTCTCTGTAATCTTTAG
Rev-GGTCTTGAAGAGCTTAGAGTGA
60 35 E-622
Pru p/du 2.02 For-AAGAATCCATCAACTGAATCC
Rev-CTACTAGGGTCTTCATCATCGG
56 40 T-271
E-315
Pru p/du 2.03 For-ACATGCGCCTCTGCGGACTA
Rev-CCATTTCTTGCACGATTCAA
60 30 T-237
E-229
Pru p/du 2.04 For-TCAGTGTCAGGCTGTCAGTG
Rev-CTCCAGATTCAGAGAGGTGC
60 35 T-267
E-235
Pru p/du 3.01 For-CACCATGCATACCCTACGTG
Rev-CGTGAGGAATCCCTAAGTGG
60 35 530, sequencing
Pru p/du 3.02 For-CTTACCCCAATGCAAATGCT
Rev-CCACTGAAACCAACAGCAGA
60 35 319, sequencing
Pru p/du 3.03 For-AGGCAGCCCTTACTTGTCCT
Rev-CCACATGCTTCTCCGTATCA
60 35 413, sequencing
Pru p/du 4.01 For-AGAAGAAATCAGAAGCAACG
Rev1-CAAGATACAACCCAGTTGC G
Rev2-CTTTGTCCCACCAAGGAATAGA
55 35 T-276
E-631
Pru du 4.02 For-AAGTACATGGTCATCCAAGC C
Rev-GTCCCTGAAATTGAAGTGTC
58 35 T-188
Pru p 4.02 For-GGCAACCACCTCTCTC CT
Rev-CAAGATACAACCCAGTTGC G
55 35 E-363
  1. 1Designed mismatching nucleotides are in bold and underlined, added pigtail bases in bold and italics.
  2. 2, Annealing temperature (Tm)
  3. 3Number of PCR cycles. In the case of two values, a touch-down PCR was performed in two steps: the first number of the "Tm" and "Cycles" column refers to the Tm and number of cycles, respectively, of the first step.
  4. 4T and E refer to parental source from 'Texas' almond and 'Earlygold' peach, respectively. Genes mapped by direct sequencing are indicated by 'sequencing'