Table 2 Markers developed and cloning and sequencing primers for mapping
From: Genomic characterization of putative allergen genes in peach/almond and their synteny with apple
| Gene | Primer sequence1 | Tm °C2 | Cycles3 | Allele length (nt)4 |
|---|---|---|---|---|
| Pru du 1.01 |
For-ATGGGTGTCTTCACATATGAGAG Rev-CTGACAACATATATACTGTAAC C | 58 | 35 | T-218 |
| Pru p 1.01 |
For-TCATACAGCTACACCTTGT T Rev-CAATGAGCTTGAAGAGATTTG | 58 | 35 | E-205 |
| Pru p/du 1.02 |
For-GTTGGAACCATCAAGAAGGAC GTTT CATGAGCATACAGAACTATC | 58 | 35 |
T-188 E-179 |
| Pru p/du 1.03 |
For-GGTGAAGGTTAGCTAGTTGA Rev-GTTT CCATCCCTGTGCTTCACAA | 58 | 35 |
T-153 E-150 |
| Pru p/du 1.04 |
For-CTTTGGTGAAGGTAAGTTTAGA Rev-GTTT CATACCTGTAAGTGCTACCTGC | 58 | 35 |
T-157 E-164 |
| Pru p/du 1.05 |
For-GCTTCCAACATCAAGTAATACCT Rev-G TTTGGGACTAGGTTGTCAGCA | 60 | 35 |
T-179 E-182 |
| Pru p/du 1.06A |
For-TAGTTATGAGTTGCTTGCAATGCT Rev-GAAAGTTCCAAAGTACATGTGC | 58 | 35 | 1871, sequencing |
| Pru du 1.06B |
For-ATCCCCAAGATTGATCCCCT G Rev-GATTAGAATTTAAGAGCTTACTGT | 60/58 | 5/35 | T-182 |
| Pru p 1.06B |
For-AAAGCCCTTGTTCTTGAAGCG Rev-GATTAGAATTTTAGAGCTTACTC C | 60/58 | 5/35 | E-212 |
| Pru p/du 1.06C |
For-CTAATTAATAACATAAATCTTGAAAC T Rev-TTGATCTCAACATCCCCTGC | 56/54 | 5/35 | E-272 |
| Pru du 2.01A |
For-CTTAGCATTCAACCAGCCT C Rev-GATGAGGTAGTCAGGGCA AG | 60 | 35 | T-175 |
| Pru p 2.01A |
For-CAATTGTCTCTGTAATCTGTTT Rev-ACGGAGCGTCCACAGAGTT | 60 | 35 | E-205 |
| Pru du 2.01B |
For-TTGCCCTGCCAATGTTAACGC Rev-GCACTGGGTCTTAAAGAACT G | 60 | 35 | T-187 |
| Pru p 2.01B |
For-CAATTGTCTCTGTAATCTTTAG Rev-GGTCTTGAAGAGCTTAGAGTGA | 60 | 35 | E-622 |
| Pru p/du 2.02 |
For-AAGAATCCATCAACTGAATCC Rev-CTACTAGGGTCTTCATCATCGG | 56 | 40 |
T-271 E-315 |
| Pru p/du 2.03 |
For-ACATGCGCCTCTGCGGACTA Rev-CCATTTCTTGCACGATTCAA | 60 | 30 |
T-237 E-229 |
| Pru p/du 2.04 |
For-TCAGTGTCAGGCTGTCAGTG Rev-CTCCAGATTCAGAGAGGTGC | 60 | 35 |
T-267 E-235 |
| Pru p/du 3.01 |
For-CACCATGCATACCCTACGTG Rev-CGTGAGGAATCCCTAAGTGG | 60 | 35 | 530, sequencing |
| Pru p/du 3.02 |
For-CTTACCCCAATGCAAATGCT Rev-CCACTGAAACCAACAGCAGA | 60 | 35 | 319, sequencing |
| Pru p/du 3.03 |
For-AGGCAGCCCTTACTTGTCCT Rev-CCACATGCTTCTCCGTATCA | 60 | 35 | 413, sequencing |
| Pru p/du 4.01 |
For-AGAAGAAATCAGAAGCAACG Rev1-CAAGATACAACCCAGTTGC G Rev2-CTTTGTCCCACCAAGGAATAGA | 55 | 35 |
T-276 E-631 |
| Pru du 4.02 |
For-AAGTACATGGTCATCCAAGC C Rev-GTCCCTGAAATTGAAGTGTC | 58 | 35 | T-188 |
| Pru p 4.02 |
For-GGCAACCACCTCTCTC CT Rev-CAAGATACAACCCAGTTGC G | 55 | 35 | E-363 |