Table 2 Markers developed and cloning and sequencing primers for mapping
From: Genomic characterization of putative allergen genes in peach/almond and their synteny with apple
Gene | Primer sequence1 | Tm °C2 | Cycles3 | Allele length (nt)4 |
|---|---|---|---|---|
Pru du 1.01 | For-ATGGGTGTCTTCACATATGAGAG Rev-CTGACAACATATATACTGTAAC C | 58 | 35 | T-218 |
Pru p 1.01 | For-TCATACAGCTACACCTTGT T Rev-CAATGAGCTTGAAGAGATTTG | 58 | 35 | E-205 |
Pru p/du 1.02 | For-GTTGGAACCATCAAGAAGGAC GTTT CATGAGCATACAGAACTATC | 58 | 35 | T-188 E-179 |
Pru p/du 1.03 | For-GGTGAAGGTTAGCTAGTTGA Rev-GTTT CCATCCCTGTGCTTCACAA | 58 | 35 | T-153 E-150 |
Pru p/du 1.04 | For-CTTTGGTGAAGGTAAGTTTAGA Rev-GTTT CATACCTGTAAGTGCTACCTGC | 58 | 35 | T-157 E-164 |
Pru p/du 1.05 | For-GCTTCCAACATCAAGTAATACCT Rev-G TTTGGGACTAGGTTGTCAGCA | 60 | 35 | T-179 E-182 |
Pru p/du 1.06A | For-TAGTTATGAGTTGCTTGCAATGCT Rev-GAAAGTTCCAAAGTACATGTGC | 58 | 35 | 1871, sequencing |
Pru du 1.06B | For-ATCCCCAAGATTGATCCCCT G Rev-GATTAGAATTTAAGAGCTTACTGT | 60/58 | 5/35 | T-182 |
Pru p 1.06B | For-AAAGCCCTTGTTCTTGAAGCG Rev-GATTAGAATTTTAGAGCTTACTC C | 60/58 | 5/35 | E-212 |
Pru p/du 1.06C | For-CTAATTAATAACATAAATCTTGAAAC T Rev-TTGATCTCAACATCCCCTGC | 56/54 | 5/35 | E-272 |
Pru du 2.01A | For-CTTAGCATTCAACCAGCCT C Rev-GATGAGGTAGTCAGGGCA AG | 60 | 35 | T-175 |
Pru p 2.01A | For-CAATTGTCTCTGTAATCTGTTT Rev-ACGGAGCGTCCACAGAGTT | 60 | 35 | E-205 |
Pru du 2.01B | For-TTGCCCTGCCAATGTTAACGC Rev-GCACTGGGTCTTAAAGAACT G | 60 | 35 | T-187 |
Pru p 2.01B | For-CAATTGTCTCTGTAATCTTTAG Rev-GGTCTTGAAGAGCTTAGAGTGA | 60 | 35 | E-622 |
Pru p/du 2.02 | For-AAGAATCCATCAACTGAATCC Rev-CTACTAGGGTCTTCATCATCGG | 56 | 40 | T-271 E-315 |
Pru p/du 2.03 | For-ACATGCGCCTCTGCGGACTA Rev-CCATTTCTTGCACGATTCAA | 60 | 30 | T-237 E-229 |
Pru p/du 2.04 | For-TCAGTGTCAGGCTGTCAGTG Rev-CTCCAGATTCAGAGAGGTGC | 60 | 35 | T-267 E-235 |
Pru p/du 3.01 | For-CACCATGCATACCCTACGTG Rev-CGTGAGGAATCCCTAAGTGG | 60 | 35 | 530, sequencing |
Pru p/du 3.02 | For-CTTACCCCAATGCAAATGCT Rev-CCACTGAAACCAACAGCAGA | 60 | 35 | 319, sequencing |
Pru p/du 3.03 | For-AGGCAGCCCTTACTTGTCCT Rev-CCACATGCTTCTCCGTATCA | 60 | 35 | 413, sequencing |
Pru p/du 4.01 | For-AGAAGAAATCAGAAGCAACG Rev1-CAAGATACAACCCAGTTGC G Rev2-CTTTGTCCCACCAAGGAATAGA | 55 | 35 | T-276 E-631 |
Pru du 4.02 | For-AAGTACATGGTCATCCAAGC C Rev-GTCCCTGAAATTGAAGTGTC | 58 | 35 | T-188 |
Pru p 4.02 | For-GGCAACCACCTCTCTC CT Rev-CAAGATACAACCCAGTTGC G | 55 | 35 | E-363 |