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Table 4 PCR primer pairs used for genomic cloning of the different peach/almond allergen genes and for (bin) mapping the genes

From: Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

Primers group Reference Sequence1 Primer name Primer sequence (5' – 3') Tm °C/cycles Product Gene
     Pfu Taq (nt)  
1 DQ251187 PpPr10f
PpPr10r
ACCATGGGTGTCTTCACATA
AATTTAGTTGTAGGCATCGG
56/30 58/2 586/623 Pru p/du 1.01/05
2 DY646736 Pp1-2f
Pp1-2r
GTTTGTCCTTTAAACTCTCC
GATTTAGTTGTAGGCATCGG
53/30 55/2 762/765 Pru p/du 1.02
3 DY647300 Pp1-3f
Pp1-3r
CTTTGATCAGTTTCCCAAGT
TTAGTTGTAGGCATCAGGGT
53/30 55/2 624 Pru p/du 1.03
4 DY653062 Pp1-4f1
Pp1-4r
TTTACAGAATCATGGGTGTG
TTAGTTGTAGGCATCATGGT
53/30 55/2 615/622 Pru p/du 1.04
5 GW1 Pp1-1A-GSP1
Pp1-1A-GSP2
AACCTTCACCAAAGCTAGTCTTCTTGATG
GTCAGCATCCAGAACAAGGGCTTTGAACA
   1100 Pru p/du 1.05
/6A/C
6 GW2 P1-1A3-GSP1
P1-1A3-GSP2
GGTGTCTTCACATACTCAGACGAGTC
CCACCTCAGTCATCCCCCCACCAAGA
   1546 Pru p/du 1.06A/C
7   P1-1-6
PpPr10r
CACCTCCAGTGCTCATAGC
See above
57/30 59/2 720 Pru p/du 1.05
8   P1-7f
P1-7r
ATGGGTGTCTTCACATACTCAG
TTTAGTTGTAGGCATCTGGGT
57/30 59/2 593 Pru p/du 1.06A/B
9   P1-8f
P1-8r2
TAGTTATGAGTTGCTTGCAATGCT
GGGCATGCTTGCTTTGGTAACTC
58/30 60/2 1346 Pru p/du 1.06C
10   P1-8f
P1-9r
See above
GAAAGTTCCAAAGTACATGTGC
58/30 60/2 1908 Pru p/du 1.06A
11 AF362988 PpTL2f
PpTL2r
ACAGCAAGCCAATTAAGACA
GCTTATGGGCAGAATGTGATGAG
56/30 58/2 879/892 Pru p/du 2.01A/B
12 AF362987 PpTL1f
PpTL1r
ATGATGAAGACCCTAGGAGCAG
GTCGAGAGTCCTATCTTTAT
56/30 58/2 974 Pru p/du 2.02
13 GW3 PTLP1-GSP1
PTLP1-GSP2
GATAATTGAGGATTTCCAAAGGAGGCTG
AGGAGGGTTAAGCTGAGGCTGAGGACTG
   500/1500 Pru p/du 2.02
14 BF717226,
DW349103
PpTLP3f
PpTLP3r
AAGTAAAGTTCTTTGGCGTC
CACGTGGCACCACAAGCATC
53/30 55/2 659 Pru p/du 2.03
15 GW4 PruTLP3-GSP1
PruTLP3-GSP2
AGTACTCACCCACCAACTTCTCCTGCGT
GCTACTTTAGCCGAGTTCACTCTCAACG
   800/1000 Pru p/du 2.03
16 Consensus
of 29 ESTs
PpTLP4f
PpTLP4r
ACTTGTCTGAACTTATGGCTCC
GAAGTTAGAAGAAGAGCTGCG
58/30 60/2 1800/1825 Pru p/du 2.04
17 AY620230
X96714
PpLTP1f1
PpLTP1r
ATCATAGTCAAGAGAGATGG
CCCTAAGTGGATCACATAGC
52/30 54/2 645 Pru p/du 3.01
18 AY093699
X96716
PpLTP2f
PpLTP2r
TATCAGCTTTACTTACGACG
CTGGCTTCCACAGAAACCTC
58/30 60/2 513/514 Pru p/du 3.02
19 X96715 PpLTP3f
PpLTP3r
CCCAAGCGAAAGAAACACTA
CATCTCATATCATCCTTCCA
58/30 60/2 527/528 Pru p/du 3.03
20 AJ491881
AY081852
Pp4.01f
Pp4.01r
AGAAGAAATCAGAAGCAACG
AAATAGTCACTCGGAGCAAT
51/30 53/2 1041 Pru p/du 4.01
21 AJ491882 Pp4.02f
Pp4.02r1
CAGCAACAACAACAAAGATG
TCTAGAGACCCTGCTCAACC
53/30 55/2 754 Pru p/du 4.02
  1. 1GW-genome walking, only gene specific primers (GSP) shown, according to sequences obtained with previous primer group, representative reference EST accessions for group 16: [GenBank:DY634569; DY644567; DY651848; DY634415]