Marker typing by qPCR and conventional method. Dissociation curve (A) and gel image (B) of DIK2849 as an example of unambiguous +/- scoring of clones by both dissociation curve and agarose gel electrophoresis in buffalo (BBU), hamster (A23) and selected RH clones. In (A), the dissociation curve is plotted as the first derivative of fluorescence relative to temperature in the SDS software view. Clones #71 (positive for BBU DNA) and #78 (negative for BBU DNA) are indicated along with hamster negative control and buffalo positive control. Peaks are easily differentiated for scoring.