Figure 5

Composition and expression patterns of significantly enriched GO categories from SAFE analysis. Panels A-D are SAFE plots for two commonly enriched categories for both deltamethrin (A & C) and permethrin (B & D). Panels E & F are SAFE plots for a category not enriched for either deltamethrin (E) or permethrin (F). The x-axis of each plot denotes the position of all probe sets in a rank ordered list of significance (from left to right) according to the empirical p-value from a linear regression across dose. The y-axis is a cumulative percentage calculated by taking the rank position of a given probe set either within the entire data set (solid unity line) or the interrogated Gene Ontology sub-category (solid stair-step line) and dividing them by the total number of probe sets contained within the entire data set or interrogated category, respectively. The degree of deviation of the stair-step line from the unity line indicates enrichment. The probe sets (excluding ESTs) that are ranked highest in significance for each GO category for both compounds in panels A-B and C-D are denoted at the top of each panel and included in a heatmap to the side of the respective panels. In the heatmaps, each row of tiles is a probe set and each column of tiles represents the mean fold-change from control with increasing doses of each compound running from right to left. Colorbar for heatmaps is given in panel G.