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Table 4 qRT-PCR confirmation of transcripts identified as dose-responsive.

From: Transcriptional response of rat frontal cortex following acute In Vivo exposure to the pyrethroid insecticides permethrin and deltamethrin

 

Deltamethrin

Permethrin

Camk1g a

qRT-PCR

1391229_at

1377518_at

qRT-PCR

1391229_at

1377518_at

Control

1.03 ± 0.10

1.00 ± 0.04

1.00 ± 0.05

1.04 ± 0.13

1.00 ± 0.04

1.00 ± 0.05

< NOAEL

1.23 ± 0.18

1.10 ± 0.03

1.07 ± 0.08

1.05 ± 0.14

1.16 ± 0.10

1.20 ± 0.13

NOAEL

1.40 ± 0.18

1.26 ± 0.07

1.31 ± 0.06

1.97 ± 0.28*

1.09 ± 0.05

1.09 ± 0.09

ED30

1.57 ± 0.14*

1.31 ± 0.06

1.39 ± 0.06

1.76 ± 0.32*

  

ED50

   

1.72 ± 0.30*

1.25 ± 0.09

1.45 ± 0.17

Ddc a

qRT-PCR

1368064_a_at

 

qRT-PCR

1368064_a_at

 

Control

1.01 ± 0.06

1.00 ± 0.03

 

1.04 ± 0.12

1.00 ± 0.03

 

< NOAEL

0.79 ± 0.06

0.96 ± 0.04

 

0.97 ± 0.10

1.04 ± 0.04

 

NOAEL

0.89 ± 0.07

0.90 ± 0.05

 

0.91 ± 0.10

1.01 ± 0.04

 

ED30

0.70 ± 0.05*

0.80 ± 0.03

 

0.81 ± 0.11*

  

ED50

   

0.71 ± 0.09*

1.00 ± 0.05

 

Gpd1 b

qRT-PCR

1371363_at

1369560_at

qRT-PCR

1371363_at

1369560_at

Control

1.06 ± 0.13

1.00 ± 0.11

1.00 ± 0.08

1.04 ± 0.14

1.00 ± 0.11

1.00 ± 0.08

< NOAEL

1.16 ± 0.13

0.88 ± 0.07

0.88 ± 0.05

0.94 ± 0.07

1.03 ± 0.10

0.98 ± 0.09

NOAEL

1.04 ± 0.12

1.42 ± 0.17

1.25 ± 0.13

0.97 ± 0.06

1.03 ± 0.09

0.95 ± 0.10

ED30

2.04 ± 0.28*

1.94 ± 0.19

1.55 ± 0.14

1.24 ± 0.15

  

ED50

   

1.23 ± 0.13

1.19 ± 0.18

1.12 ± 0.16

Fkbp51 b

qRT-PCR

1388901_at

1380611_at

qRT-PCR

1388901_at

1380611_at

Control

1.01 ± 0.06

1.00 ± 0.05

1.00 ± 0.04

1.02 ± 0.09

1.00 ± 0.05

1.00 ± 0.04

< NOAEL

0.92 ± 0.06

1.00 ± 0.03

1.04 ± 0.07

1.03 ± 0.09

0.92 ± 0.05

0.96 ± 0.07

NOAEL

1.02 ± 0.07

1.17 ± 0.05

1.16 ± 0.08

1.00 ± 0.06

1.06 ± 0.03

0.99 ± 0.06

ED30

1.52 ± 0.14*

1.41 ± 0.09

1.35 ± 0.10

0.95 ± 0.07

  

ED50

   

1.03 ± 0.12

1.07 ± 0.05

1.07 ± 0.09

c-fos c

qRT-PCR

1375043_at

 

qRT-PCR

1375043_at

 

Control

1.19 ± 0.27

1.00 ± 0.16

 

1.09 ± 0.21

1.00 ± 0.16

 

< NOAEL

0.63 ± 0.14

1.01 ± 0.31

 

1.49 ± 0.25

0.54 ± 0.07

 

NOAEL

1.09 ± 0.43

0.72 ± 0.12

 

1.30 ± 0.22

0.57 ± 0.08

 

ED30

0.54 ± 0.08*

0.69 ± 0.06

 

0.91 ± 0.11

  

ED50

   

1.25 ± 0.27

1.56 ± 0.34

 

Egr1 c

qRT-PCR

1368321_at

 

qRT-PCR

1368321_at

 

Control

1.04 ± 0.12

1.00 ± 0.08

 

1.01 ± 0.07

1.00 ± 0.16

 

< NOAEL

0.81 ± 0.05

0.93 ± 0.09

 

1.10 ± 0.10

0.77 ± 0.02

 

NOAEL

0.90 ± 0.16

0.95 ± 0.08

 

1.10 ± 0.11

0.89 ± 0.09

 

ED30

0.87 ± 0.07

0.98 ± 0.06

 

1.09 ± 0.08

  

ED50

   

1.12 ± 0.10

1.19 ± 0.08

 
  1. Values in normal type-set are 2-ΔΔCT (± SE) from qRT-PCR Cohort 3. For comparison, values in italics are fold-change from control (± SE) for microarray probe sets (Cohorts 1 & 2). Groups for statistical analysis are given in Table 1. Equipotent dose groups are based on Wolansky et al (2006). Main effects in two-way ANOVA: a = EDL (p < 0.05), b = COMPOUND*EDL (p < 0.05) and DOSE (p < 0.05) for deltamethrin only in one-way ANOVA, c = COMPOUND (p < 0.05). * = Significance difference from control in a Dunnett's mean contrast test (p < 0.05).
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BMC Genomics

ISSN: 1471-2164

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