Comparison of the Ae. speltoides sequence with the A, B D genome sequence of T. aestivum. The dot plot was performed using the DOTTER program with default parameters between Ae. speltoides gS (horizontal) and the T. aestivum gA, -gB, -gD genome (vertical) sequences. Annotation features identified for these sequences are reported on the corresponding axes. Gene numbers and names as well as color codes for TEs and other DNA sequence classes are as in figure 1. Diagonals on the dot plot output that represent nucleotide conservation between the two analyzed sequences are highlighted with dotted blue circles. The loss of micro-colinearity corresponds to diagonal breaks. 'SPA orthologous region' defined as conserved sequences between Ae. speltoides gS and T. aestivum -gA, -gB, -gD sequences are mentioned with plain arrows on the four annotation features. SPA gene is shown with dotted arrows on the dot plot out put.