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Table 1 Conserved Coding (SPA gene) and Non-coding Sequences (CNS) identified between SPA-gA-gB-gD-gS at the 'SPA orthologous region'

From: New insights into the origin of the B genome of hexaploid wheat: Evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides

Non coding 'SPA orthologous loci' sequences Coding 'SPA orthologous loci' sequences
  B D S   B D S
A     A    
CNS size (bp) 11 152 10 838 10 597 Nb of transitions 33 20 38
% Identity 85,9 87,9 86,8 Nb of transversions 22 10 23
Ks 0,874+- 0,036 1,037+-0,036 0,716+-0,024 Ratio 1,5 2 1,65
Ka 0,664+- 0,014 0,848+-0,015 0,57+-0,01 Ks 0,055+-0,015 0,042+-0,013 0,065+-0,016
Ks/Ka 1,3 1,2 1,3 Ka 0,042+-0,007 0,021+-0,005 0,049+-0,007
B     MYA 6,2–10,8 4,5–8,5 7,5–12,5
CNS size (bp)   10 666 11 976 B    
% Identity   85,8 89,9 Nb de transitions   35 25
Ks   0,991+-0,034 0,617+-0,026 Nb de transversions   20 19
Ka   0,797+-0,015 0,492+-0,012 Ratio   1,75 1,32
Ks/Ka   1,2 1,3 Ks   0,071+-0,017 0,035+-0,012
D     Ka   0,039+-0,007 0,035+-0,006
CNS size (bp)    10 039 MYA   8,3–13,5 3,5–7,2
% Identity    85,3 D    
Ks    0,902+-0,031 Nb de transitions    39
Ka    0,791+-0,014 Nb de transversions    23
Ks/Ka    1,1 Ratio    1,7
     Ks    0,089+-0,019
     Ka    0,043+-0,007
     MYA    10,8–16,6
  1. Non-coding 'SPA orthologous loci' sequences. Detailed features obtained for the 6 pairwised alignments of the 4 SPA orthologous regions excluding the SPA gene itself are mentioned with the CNS length, percentage of sequence identity, Ks and Ka values and rate. As an example, alignment of the gA and gB SPA orthologous regions of respectively 31 498 bp and 18 589 bp correspond to a cumulative CNS length of 11 152 bp with 85.9% of sequence identity and 0.874, 0.664, 1.3 values for respectively Ks, Ka and Ks/Ka.
  2. Coding 'SPA orthologous loci' sequences. 6 pairwised comparisons of the SPA gene between A/B B/D D/A A/S B/S D/S sequences are associated with the number of substitutions, the number of transition, the number of transversion, the transition/transversion ratio, the Ks value, the Ka value and speciation date (MYA).