Figure 5

Expression of the RD mRNAs in Drosophila and A. mellifera embryos. Expression of RD gene RNA in Drosophila and Honeybee embryos using in-situ hybridisation. Genes arranged by orthology based on Fig. 3. A,C,E, and G Drosophila, scale bars =100 µm B, D, F and H honeybee, scale bars= 50 µm A) run (stage 2, 5 (two focal planes), 9 and 16) is in seven stripes in blastoderm embryos, no maternal expression is seen and expression is low in later development. B) GB11654 (stages 5 and 9) is expressed in stripes in the blastoderm, and is expressed widely in later embryos. C) CG34145 (stages 2, 5 (two focal planes), 7 and 15) is expressed maternally and then ubiquitously until stage 14. D) GB19482 (stage 5 and 9) is expressed weakly in stage 5 embryos but ubiquitous later. E) CG42267 (stage 2, 5 (two focal planes), 13 and 16) is expressed maternally and then ubiquitously. F) GB15836 (stages 5 and 9) is expressed ubiquitously after blastoderm stages. G) lz (stages 3, 5 (two focal planes),11 and 15) is maternally provided localised to nuclei and ubiquitous by stage 5. H) GB16431 (stages 5 and 9) is expressed in the anterior of blastoderm embryos and ubiquitously by stage 9.