Relative quantities of origin and termini proximate DNA in exponentially growing vibrios. Relative amounts of origin and terminus proximate DNA from large (oriI and terI) and small (oriII and terII) chromosomes was determined with RT-qPCR for exponentially growing V. parahaemolyticus (Aa), V. cholerae (Ba) and V. vulnificus (Ca). Also shown are origin/terminus ratios for the separate chromosomes of V. parahaemolyticus (Ab), V. cholerae (Bb) and V. vulnificus (Cb). Bars display means for three or five experiments ± standard deviation and represent relative amounts of gDNA from cultures grown in rich broth at 37°C (light grey), from rich broth cultures at 20°C (white) and from cultures in minimal media grown at 37°C (dark grey). Large and small chromosome origin/terminus ratios were compared by paired t-tests assuming equal variances between groups and displayed significant differences (P < 0.05) for all strains and under all growth conditions.