Figure 3

Absolute quantification of the budding yeast transcriptome by large-scale GATC-PCR. (A) Reproducibility of absolute quantification of the budding yeast transcriptome by GATC-PCR. Genome-wide GATC-PCR quantification was performed twice using the same total RNA sample labeled as #2 in Table 1. (B) ''Virtual R₀t'' curve based on the merged expression data (Table 4). (C) Comparison of absolute mRNA levels between cells grown in YPD and SD media. Note that the plot includes 3,351 genes detectably expressed under both conditions but not those with undetectable levels of expression in either condition. (D) Comparison of absolute mRNA levels of genes with GO slim term ''ribosome'' between cells grown in YPD and SD media. (E) Distribution of transcript abundances in cells grown in YPD and SD media. The plot includes 3,351 genes detectably expressed in both conditions.