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Table 1 Total amount of RNAs in a single yeast cell grown in YPD medium

From: Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

Method Culture #1 Culture #2 Culture #3 Average (SD)
Hot Phenol 1.32 1.34 1.29 1.32 (0.03)
NaOH/PCA 1.43 1.45 1.43 1.43 (0.01)
  1. Total amount of cellular RNAs (pg per cell) was determined for three independent preparations of S288C cells grown in YPD medium using a modified hot-phenol method [12] and NaOH/PCA method [13].
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BMC Genomics

ISSN: 1471-2164

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