|
Name
|
Sequence
|
|---|
|
Adaptor-specific primer
|
|
M13RV
|
5' -CAGGAAACAGCTATGAC-3'
|
|
Adaptor
|
|
Forward oligonucleotide
|
|
A
|
5' -TGCACAATACTCACACAGGAAACAGCTATGACTGCGCTCACATCG-3'
|
|
B
|
5' -ACAATTCACACAGGAAACAGCTATGACTGCACTGCGCTCACATCG-3'
|
|
Reverse oligonucleotide
|
|
C
|
5' -(PO4)GATCCGATGTGAGCGCCA-3'
|
- The Y-shaped adaptor A/C or B/C was prepared by annealing equal molar amounts of the forward oligonucleotide A or B and the reverse oligonucleotide C, respectively. The sequence shared by the adaptor specific primer (ASP) and the adaptors is shown in bold. Complementary sequences between the forward and reverse oligonucleotides are underlined.
