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Table 2 Oligonucleotides used as primers and adaptors

From: Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

Name Sequence
Adaptor-specific primer
M13RV 5' -CAGGAAACAGCTATGAC-3'
Adaptor
Forward oligonucleotide
A 5' -TGCACAATACTCACACAGGAAACAGCTATGACTGCGCTCACATCG-3'
B 5' -ACAATTCACACAGGAAACAGCTATGACTGCACTGCGCTCACATCG-3'
Reverse oligonucleotide
C 5' -(PO4)GATCCGATGTGAGCGCCA-3'
  1. The Y-shaped adaptor A/C or B/C was prepared by annealing equal molar amounts of the forward oligonucleotide A or B and the reverse oligonucleotide C, respectively. The sequence shared by the adaptor specific primer (ASP) and the adaptors is shown in bold. Complementary sequences between the forward and reverse oligonucleotides are underlined.