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Table 3 Copy number of GCN4 mRNA in a single yeast cell grown in YPD medium

From: Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

Standard point

Culture #1

Culture #2

Culture #3

#1 (1 copy/cell)

ND

ND

ND

#2 (11 copies/cell)

38.7

45.4

47.3

#3 (111 copies/cell)

39.0

39.9

45.3

Average

38.9

42.7

46.3

  1. Copy number of GCN4 mRNA per cell was determined for the same samples as those in Table 1 using the competitive PCR method using a set of in vitro transcribed RNA standards shown in Figure 2B.