Standard point | Culture #1 | Culture #2 | Culture #3 |
---|
#1 (1 copy/cell) | ND | ND | ND |
#2 (11 copies/cell) | 38.7 | 45.4 | 47.3 |
#3 (111 copies/cell) | 39.0 | 39.9 | 45.3 |
Average | 38.9 | 42.7 | 46.3 |
- Copy number of GCN4 mRNA per cell was determined for the same samples as those in Table 1 using the competitive PCR method using a set of in vitro transcribed RNA standards shown in Figure 2B.