Characterization of ERE2 in Entamoeba histolytica. A) Schematic representation of ERE2. Yellow box, ERE2 open reading frame; white box, ERE2 core region; green boxes, imperfect terminal inverted repeats (TIR); stripped boxes, target site duplications (TSD). B) Multiple sequence alignment of the ERE2 5' and 3' imperfect inverted repeats and insertion sites. Bold letters represent inverted repeats; asterisks denote nucleotides conserved in both TIRs; target site duplications are shown underlined. C) Example of Eh_ERE2 transposition in a syntenic region from E. histolytica (top) and E. dispar (bottom). White boxes, protein coding genes; black box, Eh_ERE2; red boxes, LINEs. Orthologous pairs of genes are denoted by gray shading. Scaffold GenBank accession numbers are indicated on the left.