Figure 6

Secondary structure prediction of the extended LytR-CpsA-Psr domain. Full length sequences were aligned using MUSCLE and secondary structures were predicted using the prediction servers SsPro, PSIPRED, and Jpred. The consensus α-helices (red) and β-sheets (blue) based on the results of the three servers are shown for the extended LytR-CpsA-Psr domain. A schematic drawing underneath the aligned sequences further illustrates the sequential order of predicted α-helices (barrels) and β-sheets (arrows). The LytR-CpsA-Psr domain as defined in the PFAM database (PF03816) is shaded in grey. Fully conserved residues are marked by an asterisk (*) above the aligned sequences. A dash (-) represents a gap in the alignment. A colon (:) indicates that one of the following groups is conserved: STA, MILV, MILF, or NDEQ; a dot (.) indicates that one of the following groups is conserved: FVLIM, NEQHRK, SNDEQK, or SAG. The sequence identifiers correspond to the UniProt entry names (see Additional file 4), and the amino acid range covering the extended LytR-CpsA-Psr domain of each sequence is given in parentheses.