Isolation and characterization of microsatellite markers from the olive fly, Bactrocera oleae, and their cross-species amplification in the Tephritidae family

Table 3 Cross-species amplification of Bactrocera oleae microsatellite markers in other Tephritidae species

Species	Bactrocera					Anastrepha				Ceratitis		Rhagoletis
Markers	Bo	Bcu	Bco	Bd	Bt	Af	Al	Astr	Aser	Cc	Cf	Rc
Boms2	150	400	400			Sm	450/ Sm	Sm	Sm	200	200f	Sm
Boms3a	210	210	210	210	230					190	190	500
Boms3b	200	X	220	200	210	Sm	Sm	Sm	X	150		200
Boms5	110	500		700	130				300
Boms6a	150/ 400	350/ Sm		350	300/ 500			Sm	Sm		600	200/ 300
Boms6b	150										150f/ 200
Boms8	75										200
Boms10	200			180	200		180/ Sm		120f/ 150f	150	200/ 300	300
Boms11	200	Sm	220	Sm	Sm	Sm	Sm	Sm	Sm	Sm	Sm	500
Boms12	200							Sm	Sm
Boms14	150		150		150	300		Sm	Sm
Boms16	100			100	100
Boms18	171		170	170	190	Mb	Mb	Mb	Mb	300	300
Boms21	188	190	180		180
Boms22	210				500f
Boms24	200		250	250	250
Boms27	130		130	130	130	Sm	Sm	Sm	Sm	Sm	150/ Mb	Sm
Boms29	120
Boms30	150	150	150	150	150		Mb	130	130	110	110	110
Boms31	170		120	170	170	500	120f/ 300/ 500/ 600	120f	120f/ 400/ 500/ 700		800	450f
Boms32	150	150
Boms34	200		200	X	200
Boms58	180		400	150f/ 180f	150f/ 180f	400	400/ 500	500	400	Sm	Sm	Sm
Boms60	170	150f	170	170	170	150	150	150	150	170	250/ Mb	150
Boms62	174	200	160	200	180	160/ Mb	160/ Mb	160/ Mb	160/ Mb	200	160
Boms64a	150							300/ 450	500

Numbers in columns indicate PCR product size, as revealed by agarose gel electrophoresis
Sm: smear; f: faint band; Mb: multiple bands
Bo: B. oleae; Bco: B. correcta; Bcu: B. cucurbitae; Bd: B. dorsalis; Bt: B. tryoni; Cc: C. capitata; Cf: C. fasciventris; Af: A. fraterculus; Al: A. ludens; Aser: A. serpentina; Astr: A. striata; Rc: R. cerasi

ISSN: 1471-2164