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Table 4 Conservation of Bactrocera oleae microsatellite markers in Tephritidae

From: Isolation and characterization of microsatellite markers from the olive fly, Bactrocera oleae, and their cross-species amplification in the Tephritidae family

Species/

Genera

Functional primer pairs

Expected size

Presence of SSR motif

Presence of the expected motif

Mean number of uninterrupted repeats

B. oleae

29

29

29

29

100%

B. correcta

15/29 (51.7%)

13/15 (86.7%)

13/29 (44.8%)

6/7 (85.7%)

5/6 (83.3%)

5/7 (71.5%)

20/50 (40%)

B. cucurbitae

9/29 (31%)

6/9 (66.7%)

6/29 (20.7%)

2/2 (100%)

2/2 (100%)

2/2 (100%)

11/27 (40%)

B. dorsalis

14/29 (48.3%)

12/14 (85.7%)

12/29 (41.4%)

4/4 (100%)

4/4 (100%)

4/4 (100%)

29/36 (80.5%)

B. tryoni

19/29 (65.5%)

17/19 (89.5%)

17/29 (58.6%)

5/6 (83.3%)

5/5 (100%)

5/6 (83.3%)

32/53 (60%)

Bactrocera

genus

57/116 (49.1%)

48/57 (84.2%)

48/116 (41.4%)

17/19 (89.5%)

16/17 (94.1%)

16/19 (84.2%)

92/166 (55.4%)

A. fraterculus

5/29 (17.2%)

2/5 (40%)

2/29 (6.9%)

2/2 (100%)

1/2 (50%)

1/2 (50%)

8/9 (88.9%)

A. ludens

7/29 (24.1%)

5/7 (71.5%)

5/29 (17.2%)

1/1 (100%)

1/1 (100%)

1/1 (100%)

11/9 (122%)

A. serpentina

9/29 (31%)

6/9 (66.7%)

6/29(20.7%)

1/1 (100%)

1/1 (100%)

1/1 (100%)

8/9 (88.9%)

A. striata

7/29 (24.1%)

5/7 (71.5%)

5/29 (17.2%)

1/1 (100%)

1/1 (100%)

1/1 (100%)

12/9 (133%)

Anastrepha genus

28/116 (24.1%)

18/28 (64.3%)

18/116 (15.5%)

5/5 (100%)

4/5 (80%)

4/5 (80%)

39/36 (108.3%)

C. capitata

8/29 (27.6%)

7/8 (87.5%)

7/29 (24.1%)

1/2 (50%)

1/1 (100%)

1/2 (50%)

2/3 (66.7%)

C. fasciventris

12/29 (41.4%)

9/12 (75%)

9/29 (31%)

2/2 (100%)

2/2 (100%)

2/2 (100%)

12/24 (50%)

Ceratitis genus

20/58 (34.5%)

16/20 (80%)

16/58 (27.6%)

3/4 (75%)

3/3 (100%)

3/4 (75%)

15/27 (55.5%)

R. cerasi

8/29 (27.6%)

4/8 (50%)

13.8%

1/3 (33.3%)

1/1 (100%)

1/3 (33.3%)

2/3 (66.7%)

  1. Functional primer pairs: number of primer pairs that cross-amplified successfully; Expected size: number of primer pairs that cross-amplified and produced fragment of the expected size (calculated either in regard to total available primer pairs or in regard to primer pairs that successfully cross-amplified); Presence of SSR motif: number of cross-species amplification products that harboured a microsatellite motif; Presence of the expected motif: number of cross-species amplification products that harboured the expected microsatellite motif (calculated either in regard to total sequencing reactions or in regard to cross-species amplification products that harboured a microsatellite motif); Mean number of uninterrupted repeats: sum and comparison of the number of uninterrupted repeats for primer pairs that cross-amplified and harboured the expected motif. Dividend represents repeats in the new species, while divider stands for number of repeats in B. oleae
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BMC Genomics

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