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Table 4 Conservation of Bactrocera oleae microsatellite markers in Tephritidae

From: Isolation and characterization of microsatellite markers from the olive fly, Bactrocera oleae, and their cross-species amplification in the Tephritidae family

Species/
Genera
Functional primer pairs Expected size Presence of SSR motif Presence of the expected motif Mean number of uninterrupted repeats
B. oleae 29 29 29 29 100%
B. correcta 15/29 (51.7%) 13/15 (86.7%)
13/29 (44.8%)
6/7 (85.7%) 5/6 (83.3%)
5/7 (71.5%)
20/50 (40%)
B. cucurbitae 9/29 (31%) 6/9 (66.7%)
6/29 (20.7%)
2/2 (100%) 2/2 (100%)
2/2 (100%)
11/27 (40%)
B. dorsalis 14/29 (48.3%) 12/14 (85.7%)
12/29 (41.4%)
4/4 (100%) 4/4 (100%)
4/4 (100%)
29/36 (80.5%)
B. tryoni 19/29 (65.5%) 17/19 (89.5%)
17/29 (58.6%)
5/6 (83.3%) 5/5 (100%)
5/6 (83.3%)
32/53 (60%)
Bactrocera
genus
57/116 (49.1%) 48/57 (84.2%)
48/116 (41.4%)
17/19 (89.5%) 16/17 (94.1%)
16/19 (84.2%)
92/166 (55.4%)
A. fraterculus 5/29 (17.2%) 2/5 (40%)
2/29 (6.9%)
2/2 (100%) 1/2 (50%)
1/2 (50%)
8/9 (88.9%)
A. ludens 7/29 (24.1%) 5/7 (71.5%)
5/29 (17.2%)
1/1 (100%) 1/1 (100%)
1/1 (100%)
11/9 (122%)
A. serpentina 9/29 (31%) 6/9 (66.7%)
6/29(20.7%)
1/1 (100%) 1/1 (100%)
1/1 (100%)
8/9 (88.9%)
A. striata 7/29 (24.1%) 5/7 (71.5%)
5/29 (17.2%)
1/1 (100%) 1/1 (100%)
1/1 (100%)
12/9 (133%)
Anastrepha genus 28/116 (24.1%) 18/28 (64.3%)
18/116 (15.5%)
5/5 (100%) 4/5 (80%)
4/5 (80%)
39/36 (108.3%)
C. capitata 8/29 (27.6%) 7/8 (87.5%)
7/29 (24.1%)
1/2 (50%) 1/1 (100%)
1/2 (50%)
2/3 (66.7%)
C. fasciventris 12/29 (41.4%) 9/12 (75%)
9/29 (31%)
2/2 (100%) 2/2 (100%)
2/2 (100%)
12/24 (50%)
Ceratitis genus 20/58 (34.5%) 16/20 (80%)
16/58 (27.6%)
3/4 (75%) 3/3 (100%)
3/4 (75%)
15/27 (55.5%)
R. cerasi 8/29 (27.6%) 4/8 (50%)
13.8%
1/3 (33.3%) 1/1 (100%)
1/3 (33.3%)
2/3 (66.7%)
  1. Functional primer pairs: number of primer pairs that cross-amplified successfully; Expected size: number of primer pairs that cross-amplified and produced fragment of the expected size (calculated either in regard to total available primer pairs or in regard to primer pairs that successfully cross-amplified); Presence of SSR motif: number of cross-species amplification products that harboured a microsatellite motif; Presence of the expected motif: number of cross-species amplification products that harboured the expected microsatellite motif (calculated either in regard to total sequencing reactions or in regard to cross-species amplification products that harboured a microsatellite motif); Mean number of uninterrupted repeats: sum and comparison of the number of uninterrupted repeats for primer pairs that cross-amplified and harboured the expected motif. Dividend represents repeats in the new species, while divider stands for number of repeats in B. oleae