Figure 5

Core TRRs analysis of the OsALYL1 gene and experimental verifications. (A) The x-axis represents the genomic positions of the OsALYL1 (AK064472) gene, and the y-axis represents the core TRR scores from the HSL model using tuple-pair dictionary from S. cerevisiae. Significant TRR signals are observed immediate upstream of the TSS of OsALYL1 and in intron 11 of OsALYL1. (B) The OsALYL1 5'-upstream putative promoter region, which contains ~1.2 kb upstream of the coding region, and the full length of its intron 11 were fused to the GUS reporter gene with the nopaline synthase terminator and cloned into the binary vector pCAMBIA1303, respectively. The constructions were named as pALYL1::GUS and pIntron11::GUS, respectively. The GUS staining (blue) was observed at the node of the transgenic lines under the control of both pALYL1::GUS and pIntron11::GUS. This observation suggests that the predicted sequences possess promoter activities in both the upstream of the coding region and intron 11.