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Table 3 Real-Time PCR results of 16 genes belonging to the p21 pathway compared to the fold change (FC) detected by Affymetrix microarrays.

From: Analysis of the retinal gene expression profile after hypoxic preconditioning identifies candidate genes for neuroprotection

Gene symbol

FC Affymetrix Chip

FC PCR

Cdkn1a (p21)

30.5

20.3*

Sema3c

5.9

3.9*

Egf

4.7

2.8*

HMGB2

4.1

3.0*

Fabp4

3.5

5.3*

CEBPd

3.5

3.5*

Timp3

3.3

3.7*

ID1

3.1

1.8*

Rad23b

2.6

1.2

Vegf

2.3

2.0*

Stom

2.0

1.3

Hes6

1.9

1.6*

SOS1

1.9

0.9

Stat1

0.5

1.1

Thra

0.5

0.8

IBRDC2

0.3

0.9

  1. Analysis of gene expression was immediately after hypoxia
  2. FC: fold change. * p < 0.05
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BMC Genomics

ISSN: 1471-2164

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