Table 1 Success rate for the amplification of target sequences in multiplex-ready and conventional multiplex PCR assays
From: Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping
| Â | Â | Â | Number of primer sets successfully amplified | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | # assays | 6 | 5 | 4 | 3 | 2 | 1 | 0 |
Multiplexed SSR Assays | Â | Â | Â | Â | Â | Â | Â | Â | Â |
6-plex PCRs | Apricot | 32 | 17 (53%) | 10 (31%) | 3 (10%) | 1 (3%) | 1 (3%) | . | . |
| Â | Cherry | 32 | 19 (59%) | 9 (28%) | 4 (13%) | . | . | . | . |
| Â | Barley | 48 | 19 (40%) | 19 (40%) | 4 (8%) | 4 (8%) | 2 (4%) | . | . |
| Â | Bread wheat | 48 | 13 (27%) | 18 (38%) | 8 (17%) | 7 (14%) | 1 (2%) | 1 (2%) | . |
4-plex PCRs | Barley | 32 | . | . | 24 (75%) | 6 (19%) | 2 (6%) | . | . |
| Â | Bread wheat | 32 | . | . | 22 (69%) | 10 (31%) | . | . | . |
Multiplexed Barley SNP Assays | Â | Â | Â | Â | Â | Â | Â | Â | |
6-plex PCRs | Multiplex-Ready | 24 | 6 (25%) | 10 (42%) | 5 (21%) | 3 (12%) | . | . | . |
| Â | Conventional | 24 | . | . | 3 (12%) | 5 (21%) | 11 (46%) | 3 (12%) | 2 (8%) |
4-plex PCRs | Multiplex-Ready | 24 | . | . | 19 (79%) | 5 (21%) | . | . | . |
| Â | Conventional | 24 | . | . | 3 (12%) | 9 (38%) | 7 (29%) | 5 (21%) | . |