Figure 8

Tissue-specific qualitative expression of the barramundi MSTN-1 gene. Reverse transcriptase PCR was conducted for 30 cycles (30×) and 35 cycles (35×). Lanes are represented as follows: Marker; E, eye; G, gill; H, heart; B, brain; M, muscle; Go, gonad; I, intestine; S, spleen; K, kidney; L, liver; C, negative control. As a positive control of the RT-PCR reaction step, a 94 bp fragment was successfully amplified in 18S rRNA.